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Effects of vitamin E deficiency on the endocrine glands especially on the function of pituitary and testis.

维生素E缺乏对内分泌腺尤其是垂体和睾丸功能的影响。

基本信息

批准号：
61580054
负责人：
AKAZAWA Noriko
金额：
$ 1.28万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61580054/
关键词：
Vitamin E deficiency Pituitary-gonadal function FSH and LH secretion Testosterone secretion LH receptor 精巣抗酸化剤テストステロン FSHとLHレセプター

项目摘要

Chronological changes of gonadotropin(FSH and LH) and testosterone concentration in the serum were measured in vitamin E deficient rats to investigate the effects of vitamin E deficiency on the pituitary-gonadal function in rats. The receptor sites and association constant(Ka) for LH and the formation of cyclic AMP in the Leyding cells were also investigated. The results obtained in this research are as follows :1) The vitamin E deficient rats showed almost complete hemolysis and extremely increased TBA reacting substance in the serum and liver.2) The serum LH concentration in the vitamin E deficient group was slightly higher than in the vitamin E supplemented group during the later periods of experiment.3) The serum FSH concentration in the vitamin E deficient group became significantly higher than in the vitamin E supplemented group at 196 days of experiment.4) The serum testosterone concentration was always lower in the vitamin E deficient group than in the control. However, the decrease of testosterone in plasma and tissue was prevented by the administration of DPPD.5) The vitamin E deficient group showed slightly large number of LH/hCG receptor and significantly small Ka (low affinity), as compared with vitamin E supplemented group. The formation of cyclic AMP by Leydig cells decreased significantly in vitamin E deficient group. These results suggest that the vitamin E deficiency exerted a suppressive effect directly on the gonadal function to decrease the hormone synthesis in the Leydig cells and caused the increased secretion of pituitary LH owing to the feedback mechanism.

本实验观察了维生素E缺乏大鼠血清促性腺激素（FSH、LH）和睾酮浓度的动态变化，以探讨维生素E缺乏对大鼠垂体-性腺功能的影响。并对Leyding细胞中LH的受体位点和结合常数（Ka）以及cAMP的形成进行了研究。结果表明：1）维生素E缺乏大鼠几乎完全溶血，血清和肝脏中TBA反应物显著增加; 2）维生素E缺乏组血清LH浓度在实验后期略高于维生素E补充组; 3）维生素E缺乏组血清FSH浓度在实验第196天显著高于维生素E补充组; 4）维生素E缺乏组血清睾酮浓度始终低于对照组。5）与维生素E补充组相比，维生素E缺乏组的LH/hCG受体数量略多，Ka值（低亲和力）显著降低。维生素E缺乏组睾丸间质细胞环腺苷酸的生成明显减少。结果提示，维生素E缺乏可直接抑制性腺功能，使睾丸间质细胞激素合成减少，并通过反馈机制引起垂体LH分泌增加。