权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genes involved in intercellular signal transduction during melanocyte differentiation in the mouse.

小鼠黑素细胞分化过程中参与细胞间信号转导的基因。

基本信息

批准号：
62540480
负责人：
TAKEUCHI Takuji
金额：
$ 1.02万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

A signal transduction mechanism in the cell membrance, which receive information from the cellular environment and transfers it to the site of cellular function, has been demonstrated in various cell types. At the final step of melanocyte differentiation in mouse hair follicles, the cells produce melanin. The type of melanin they produce is, however, determined by the tissue. environment of hair follicles. In wild-type mice, melanocytes located in hair bulbs synthesize eumelanin at the beginning of hair growth. They subsequently produce pheomelanin and finally produce eumelanin again. Therefore, the hair is characterized by a subterminal band of yellow, with the rest of it displaying black. This characteristic is called the agouti pattern and is known to be determined by the wild-type allele, A__-, at the a__-(agouti) locus, which is considered to function in the dermal cells. By using an organ culture method, we have demonstrated that the product of the a__- locus (A factor) blocks th … More e action of MSH (melanocyte stimulating hormone) at the・MSH receptor site, and modifies the pathway of melanin biosynthesis. An attempt was made in the present study to clone cDNA for the A factor.Mice homozygous for a__- allele were injected with skin homogenate from the mouse, genotype A^y/a__-, which constitutively produces the A factor. Polyclonal as well as monoclonal antibodies were prepared from these mice. Immunohistochemical localization of the antigen revealed that cells scatterd in dermis were positive. Thses cells, presumably mast cells, ate expected to be the site of the gene action of the a__- locus from the results of previous studies. A cDNA library was constructed from poly (A)^+mRNA from the mouse skin with the genotype A^y/a using gtll vector, and screened with the antibodies. Two clones, A^y11 (2.8 kbp)and A^y9(750 bp), were isolated. Results of the in situ hybridization by using the cDNAs as probes demonstrated that the mRNA hybridized with the A^y11 was located in the dermal cells (presumably mast cells) and that the site of the transcription for A^y9 was melanocyte. Therefore, it is likely that the A^y11 is a cDNA for the A factor and that the A^y9 is a cDNA for a product of secondary gene action induced by the A factor. Less

细胞膜中的信号转导机制，即从细胞环境接收信息并将其传递到细胞功能部位，已在各种细胞类型中得到证实。在小鼠毛囊中黑素细胞分化的最后一步，细胞产生黑色素。然而，它们产生的黑色素的类型是由组织决定的。毛囊的环境在野生型小鼠中，位于毛球中的黑素细胞在毛发生长开始时合成真黑素。它们随后产生褐黑素，最后再次产生真黑素。因此，头发的特点是由一个黄色的亚末端带，其余部分显示黑色。这种特征被称为聚集模式，已知是由a__-（聚集）基因座上的野生型等位基因A__-决定的，该基因座被认为在真皮细胞中起作用。通过器官培养方法，我们证明了a__-位点的产物（A因子）阻断了 ...更多信息通过调节黑素细胞刺激激素（MSH）受体位点的作用，改变黑素生物合成途径。本研究试图克隆A因子的cDNA，将基因型为A^y/a__-的小鼠的皮肤匀浆注射到a__-等位基因纯合子小鼠中，该小鼠组成性产生A因子。从这些小鼠制备多克隆以及单克隆抗体。免疫组织化学定位显示真皮内散在的细胞呈阳性。这些细胞，可能是肥大细胞，从以前的研究结果来看，被认为是a__-位点的基因作用位点。以基因型A^y/a的小鼠皮肤为材料，利用gtll载体构建poly（A）^+mRNA cDNA文库，并进行抗体筛选。分离到两个克隆，A^y11（2.8 kbp）和A^y9（750 bp）。以cDNA为探针的原位杂交结果表明，与A^y11杂交的mRNA位于真皮细胞（推测为肥大细胞）中，而A^y9的转录位点是黑素细胞。因此，很可能A^y11是A因子的cDNA，而A^y9是A因子诱导的次级基因作用产物的cDNA。少