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Studies on oocyte growth by the chimera frog of diploid and triploid

二倍体和三倍体嵌合蛙卵母细胞生长的研究

基本信息

批准号：
62540548
负责人：
AMANO Minoru
金额：
$ 0.83万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62540548/
关键词：
chimera frog triploid oocyte gonad oocyte growth 卵母細胞発育不良生殖綿 3倍対

项目摘要

Triploid frog, Xenopus laevis can be produced by the cold treatment after fertilization. The oocyte in triploid ovary can not grow and does not become ooxocyte. Chimera frog between diploid and triploid germ cell was produced to study the relationship of germ cell and other mesenchymal tissue in ovary in relation to oocyte growth.1. Primordial germ cell containing area was cut out as a block and exchanged between diploid and triploid embryos at early tail bud stage. The operated embryos were seems to be normal in external morphology and their gonad were studied histologically at 5 month after metamorphosis.2. Primordial germ cell exchanged embryos between diploid one were produced as the control experiment of operation effect. Their gonads were normal and exactly same as those of not-operated diploid frogs. These results showed that exchange operation of primordial germ cell did not affect on the growth of oocyte.3. The triploid frogs contained diploid germ cells showed many grown oocytes in their ovaries. These results indicated that diploid oocytes can grow in triploid environment.4. The ovaries of diploid frogs contained triploid germ cells were different from those of normal diploid ones and similar to triploid frog ovaries. In other words, the triploid germ cells did not grow in diploid ovary and systemic structures. These results mentioned above suggest that the growth arrest of triploid oocytes are due to the triploidy of germ cells.

受精后经冷处理可产生三倍体非洲爪蟾。三倍体卵巢中的卵母细胞不能生长，不能成为卵母细胞。制备了二倍体和三倍体生殖细胞嵌合体蛙，研究卵巢生殖细胞和其他间充质组织与卵母细胞生长的关系。在尾芽期早期，将原始生殖细胞含区切成块，在二倍体和三倍体胚胎之间进行交换。胚胎外部形态正常，变态后5个月对其性腺进行组织学观察。制备了二倍体间的原始生殖细胞交换胚，作为操作效果的对照实验。它们的性腺是正常的，与未手术的二倍体青蛙完全相同。结果表明，原始生殖细胞的交换操作对卵母细胞的生长没有影响。含有二倍体生殖细胞的三倍体青蛙卵巢内有许多成熟的卵母细胞。这些结果表明二倍体卵母细胞可以在三倍体环境中生长。含有三倍体生殖细胞的二倍体蛙卵巢与正常二倍体蛙卵巢不同，与三倍体蛙卵巢相似。换句话说，三倍体生殖细胞不能在二倍体卵巢和系统结构中生长。上述结果表明，三倍体卵母细胞的生长停滞是由于生殖细胞的三倍体所致。