Synthetic Studies on organic Reagents with Specific fluorescence and Their Application to Kinetic Analysis

特异性荧光有机试剂的合成研究及其在动力学分析中的应用

• 批准号: 62550544
• 负责人: MURATA Akira
• 金额: $ 1.15万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62550544/
• 关键词: organic fluorescence reagent; spectrofluorimetry; 反応速度の差を用いる分析; 蛍光比色分析

From the theromdynamic and the kinetic studies on the reactions involving a metal complex with coumarines, chromones or flavones as a fluorescence ligand, the following results which provide a basis for the sensitive and the selective determination of the species are obtained:1.From the fluorimetric studies on the inclusion reactions of 3-hyroxyflavones and their tungsten(VI) and molybdenum(VI) complexes into cyclodextrine, a detailed picture of the thermodynamics and the kinetics of the fluorescence enhancement reaction has been obtained.2. From the kinetic studies on the oxidation reaction of coumarines by periodate, the acceleration effect of a metal ion was quantitatively accounted for. Based on the difference in the catalytic effect, a kinetic mothod for the determination in the range of 2-60 ppb has been proposed.3. From the thermodynamic studies on the complexation reactions of antimony(III) and (V) with chromones and flavones, a spectrophotmetric method for the simultaneous determination of antimony(III) and (V) with an error about 1 g has been proposed.4. From a mechanistic consideration on the reactivity of a metal ion and its complex, the perameters characteristic of a metal ion and a ligand have been estimated. With these parameters, some possibilities to design a new kinetic-catalytic of analysis based on ligand substitution reactions have been proposed.

通过对香豆素类、色酮类和黄酮类金属络合物荧光配体包结反应的热力学和动力学研究，获得了如下结果，为灵敏和选择性地测定这些物质提供了依据：1.通过对3-羟基黄酮及其钨（VI）、钼（VI）络合物与环糊精包结反应的荧光分析，得到了详细的荧光增强反应热力学和动力学图.从高碘酸盐氧化香豆素反应的动力学研究中，定量地解释了金属离子的加速作用。根据催化效应的差异，提出了在2 - 60 ppb范围内测定的动力学方法.通过对锑（Ⅲ）和锑（Ⅴ）与色酮和黄酮络合反应的热力学研究，提出了一种同时测定锑（Ⅲ）和锑（Ⅴ）的光度法，误差约为1 g.从金属离子及其配合物反应性的机理考虑，估算了金属离子和配体的特征参数。在此基础上，提出了基于配体取代反应设计新的动力学催化分析方法的可能性。

项目成果

伊東琢史: 分析化学.

伊藤隆：分析化学。

山田真吉: Mikrochim.Acta [Wien]. III. 291-297 (1988)

Shinkichi Yamada：Mikrochim.Acta [维也纳] III。

MURATA,Akira: "Spectrophotometric Determinatioin of Antimony(III) and (V) with 3,7-Dihydroxy-flavone and Quercetin"

MURATA，Akira：“用 3,7-二羟基黄酮和槲皮素分光光度法测定锑 (III) 和 (V)”

山田真吉: Mikrochim.Acta[Wien]. III. 291-297 (1988)

Shinkichi Yamada：Mikrochim.Acta[维也纳] III。

YAMADA, Shinkichi: "Effect of Cyclodextrin on the Fluorescence Reactions Involving 3-Hydroxyflavones"

YAMADA, Shinkichi：“环糊精对涉及 3-羟基黄酮的荧光反应的影响”