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Structure Elucidation, Nutrition and Analytical Methods of Vitamin B_6 Conjugates in Plant Foods.

植物性食品中维生素 B_6 结合物的结构解析、营养和分析方法。

基本信息

批准号：
62560090
负责人：
TADERA Kenjiro
金额：
$ 1.28万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Grains and legumes are regarded as good sources of vitamin B_6 (B_6) for Japanese. B_6 in plant foods, however, is incompletely available to animals because it occurs mainly as bound forms. Reports on the structures of the bound forms are scarce except for 5'-O-( -d-glucopyranosyl)-pyridoxine (PNG).1) Three new B_6 glycosides (A, B and C) were isolated from rice bran (10 kg) by various chro-matographic techniques. A, B, and C were shown to consist of pyridoxine (PN) and glucose (GLc) in 1:2, 1:3 and 1:3 molar ratios, respectively, by -glucosidase hydrolysis and Si-MS data. Gibbs test and ^<13>C-NMR spectral data revealed that A was 5'-O-( -cellobiosyl)pyridoxine (PNC). From the results of the Gibbs color reaction and partial hydrolyses which yielded PNC, B was concluded to be 4'-O-( -D-glucosyl)-5'-O-( b-cellobiosyl)pyridoxine, and C to be 5'-O-( -glucotriosyl)pyridoxine.2) A new type of B_6 conjugate was isolated from rice bran (11.4 kg) by adsorption, ion-exchange and gel-filtration methods. The compound gave PN and Glc by alkaline treatment followed by -glu-cosidase hydrolysis. H-NMR, UV and IR spectral data showed that it was an ester of PNC. The acid (MW, 223) seemed to be a kind of aromatic acid. Its structure is being examined.3) a paired-ion reverse-phase high-performance liquid chromatography was developed for the deter-mination of six B_6 vitamers, pyridoxic acid and PNG, a main B_6 component in plant foods. This procedure showed high reproducibility and could detect 0.5 - 1 ng of these compounds. It was found suitable as a routine procedure for the analysis of animal-and plant-foods within 40 min. The bioavailability of the compounds isolabed was assumed to be comparable bo that of PNG.

谷物和豆类被认为是日本人维生素B_6（B_6）的良好来源。然而，植物性食物中的B_6对动物来说并不完全有效，因为它主要以结合形式存在。1）从米糠（10 kg）中分离得到三个新的B_6糖苷（A、B和C）。A，B，和C被证明是由吡哆醇（PN）和葡萄糖（GLc）在1：2，1：3和1：3的摩尔比，分别由β-葡萄糖苷酶水解和Si-MS数据。Gibbs实验和~ 1C-NMR<13>谱数据表明A为5 ′-O-（β-纤维二糖基）吡哆醇（PNC）。根据吉布斯显色反应和部分水解产物PNC的结果，确定B为4 ′-O-（-D-葡萄糖基）-5 ′-O-（β-纤维二糖基）吡哆醇，C为5 ′-O-（-葡萄糖三糖基）吡哆醇。该化合物经碱处理后，再经β-葡萄糖苷酶水解得到PN和Glc。通过核磁共振氢谱、紫外光谱和红外光谱分析，证明它是PNC的酯。分子量为223的酸似乎是一种芳香酸。3）建立了一种双离子反相高效液相色谱法测定植物性食品中六种维生素B_6、吡哆酸和主要维生素B_6组分PNG的方法。该方法具有较高的重现性，可检测0.5 - 1 ng的这些化合物。它被认为是合适的作为一个常规程序的动物和植物食品的分析在40分钟内。分离的化合物的生物利用度被认为是可比博的PNG。