Pepstatin-Insensitive Carboxyl Proteinase : Glutamic Proteinase

胃酶抑素不敏感的羧基蛋白酶:谷氨酸蛋白酶

基本信息

  • 批准号:
    62560112
  • 负责人:
  • 金额:
    $ 1.15万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1987
  • 资助国家:
    日本
  • 起止时间:
    1987 至 1989
  • 项目状态:
    已结题

项目摘要

It is well known that carboxyl proteases are commonly inhibited by pepstatin^<1)> , DAN^<2)> and EPNP^<3)> , and their catalytic residues are composed of two aspartic residues. Thus, carboxyl proteases are termed aspartic proteases. These enzymes are highly homologous in both the primary and tertiary structures.We have isolated novel carboxyl proteases from fungi, bacteria and also thermophilic bacteria based on their insensitivities to pepstatin, DAN and EPNP. These enzymes were tentatively named pepstatin-insensitve carboxyl proteases. In one of our studies, the primary structure of carboxyl protease B ( consisting of 204 aminb acids) from a fungus Scytalidium lignicolum has been established, one of the catalytic residues of which was clarified to be Glu-53. This is the first report on glutamic protease.It seemed probable that the pepstatin-insensitive carboxyl proteases are not aspartic proteases but glutamic proteases. To confirm this possibility, we studied a pepstatin-insensitive … More carboxyl protease from Pseudomonas sp. No. 101, which is the the first carboxyl protease isolated from prokaryote cells. We determined the primary structure of the enzyme as a single polypeptide composed of 363 amino acid residues with one disulfide bridge. The elucidated primary structure does not have any homologous structure to those of aspartic proteases reported so far. Moreover, the well-conserved structure, -Asp*-Thr-Gly- in the active center of aspartic proteases was not observed.In our attempt to use inhibitor in the study of active center we isolated a novel inhibitor from Kitasatosporia sp. No. 55. It was identified as N-isovaleryl-tyrosyl-leucyl-tyrosinal and named it tyrostatin. Tyrostatin was found to inhibit stoichiometrically pepstatin- insensitive carboxyl proteinases of Pseuomonas sp. No. 101.Further study on identification of the catalytic residues of Pseudomonas carboxyl protease using tyrostatin or its derivatives is now in progress. 1 ) pepstatin, pepsin inhibitor; 2) DAN, diazoacetyl-DL-norleucine methylester; 3) EPNP, 1,2-epou-3-(p- nitrophenoxy) propane. Less
众所周知,羧基蛋白酶通常被胃蛋白酶抑制剂^(1)>、DAN^(2)>和EPNP^(3)>抑制,并且它们的催化残基由两个天冬氨酸残基组成。因此,羧基蛋白酶被称为天冬氨酸蛋白酶。这些酶在一级结构和三级结构上高度同源。我们从真菌、细菌和嗜热细菌中分离出了新的羧基蛋白酶,基于它们对胃蛋白酶抑制剂、DAN和EPNP不敏感。这些酶暂命名为胃蛋白酶抑制素不敏感的羧基蛋白酶。在我们的一项研究中,已经确定了来自真菌Scytalidium ligniculum的羧基蛋白酶B(由204个氨基B酸组成)的一级结构,其中一个催化残基被澄清为Glu-53。这是首次报道谷氨酸蛋白酶,胃蛋白酶抑制剂不敏感的羧基蛋白酶很可能不是天冬氨酸蛋白酶,而是谷氨酸蛋白酶。为了证实这种可能性,我们研究了一种胃蛋白酶抑制素不敏感的 ...更多信息 假单胞菌101号的羧基蛋白酶,这是第一个从原核细胞中分离的羧基蛋白酶。我们确定的酶的一级结构作为一个单一的多肽组成的363个氨基酸残基与一个二硫键。所阐明的一级结构与迄今为止报道的天冬氨酸蛋白酶没有任何同源结构。另外,在天冬氨酸蛋白酶活性中心没有发现保守的结构-Asp*-Thr-Gly-。为了利用抑制剂研究天冬氨酸蛋白酶活性中心,我们从北中孢菌(Kitasatosporia sp.No.55)中分离到一种新的抑制剂。经鉴定为N-异戊酰-酪氨酰-亮氨酰-酪氨酸醛,命名为酪氨酸抑制素。酪蛋白抑制素对假单胞菌101的胃蛋白酶抑制素不敏感的羧基蛋白酶具有化学计量的抑制作用。目前,利用酪蛋白抑制素或其衍生物鉴定假单胞菌羧基蛋白酶催化残基的研究正在进行中。1)胃蛋白酶抑制剂,胃蛋白酶抑制剂; 2)DAN,重氮乙酰基-DL-正亮氨酸甲酯; 3)EPNP,1,2-环氧-3-(对硝基苯氧基)丙烷。少

项目成果

期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kohei Oda: Agric.Biol.Chem.51. 3073-3080 (1987)
小田耕平:Agric.Biol.Chem.51。
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    0
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  • 通讯作者:
E. Majima, K. Oda, S. Murao and E. Ichishima: "Comparative Study on the Specificities of Several fungal Aspartic and Acid Proteinases towards the Tetradecapeptide of a Renin Substrate" Agric. Biol. Chem., 52, 787-793, 1988.
E. Majima、K. Oda、S. Murao 和 E. Ichishima:“几种真菌天冬氨酸和酸性蛋白酶对肾素底物十四肽的特异性的比较研究”Agric。
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    0
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K.Oda,T.Nakazima,T.Terashita,K.Suzuki and S.Murao: "Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp.Bacterium" Agric.Biol.Chem.51. 3073-3080 (1987)
K.Oda、T.Nakazima、T.Terashita、K.Suzuki 和 S.Murao:“来自黄单胞菌属细菌的 S-PI(胃酶抑素 Ac)不敏感羧基蛋白酶的纯化和特性”Agric.Biol.Chem。
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  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
K.Oda,Y.Fukuda,S.Murao,K.Uchida and M.Kainosho: "A Novel Proteinase Inhibitor,Tyrostatin,Inhibiting Some Pepstatin-insensitive Carboxyl Proteinases" Agric.Biol.Chem.53. 405-415 (1989)
K.Oda、Y.Fukuda、S.Murao、K.Uchida 和 M.Kainosho:“一种新型蛋白酶抑制剂,酪抑素,抑制一些胃抑素不敏感的羧基蛋白酶”Agric.Biol.Chem.53。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K. Oda, Y. Fukuda, S. Murao, K. Uchida and M. Kainosho: "A Novel Proteinase Inhibitor, Tyrostatin, Inhibiting Some Pepstatin-insensitive Carboxyl Proteinases" Agric. Biol. Chem., 53, 405-415, 1989.
K. Oda、Y. Fukuda、S. Murao、K. Uchida 和 M. Kainosho:“一种新型蛋白酶抑制剂,酪抑素,可抑制某些胃抑素不敏感的羧基蛋白酶”Agric。
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    0
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ODA Kohei其他文献

Writing of two dimensional crystal curved lines in Sm_2O_3-Bi_2O_3-B_2O_3 glass by samarium atom heat processing
钐原子热处理在Sm_2O_3-Bi_2O_3-B_2O_3玻璃中写入二维晶体曲线
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    AOKI Kaoru;DATE Yusuke;TANAKA Daiki;ODA Kohei;R.Ihara
  • 通讯作者:
    R.Ihara
Characteristics of BCN compound as electrode material
BCN化合物作为电极材料的特性
Characteristics of BCN compound derived from melamine diborate
三聚氰胺二硼酸盐衍生的BCN化合物的特性

ODA Kohei的其他文献

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{{ truncateString('ODA Kohei', 18)}}的其他基金

Biochemical characterization of human CLN2, related to a fatal neurodegenerative disease : On the basis of the discovery of a novel family of peptidases
与致命的神经退行性疾病相关的人类 CLN2 的生化特征:基于新型肽酶家族的发现
  • 批准号:
    15380072
  • 财政年份:
    2003
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Microbial carboxyl proteinases related to a fatal neurodegenerative disease: proposal for a novel catalytic mechanism
与致命性神经退行性疾病相关的微生物羧基蛋白酶:提出一种新的催化机制
  • 批准号:
    13460043
  • 财政年份:
    2001
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Novel Carboxyl Proteinases : Structure, Function, and Evolution
新型羧基蛋白酶:结构、功能和进化
  • 批准号:
    11694206
  • 财政年份:
    1999
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Structure-Function, and Molecular Evolution of NCL disease-related Novel Carboxyl Proteinases from Bacteria
与 NCL 疾病相关的细菌新型羧基蛋白酶的结构功能和分子进化
  • 批准号:
    11660090
  • 财政年份:
    1999
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Structure-Function Relationships and Molecular Evolutions of Novel Carboxyl Proteinases from Microorganisms
微生物新型羧基蛋白酶的结构功能关系和分子进化
  • 批准号:
    09660089
  • 财政年份:
    1997
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Structure-Function of Novel Carboxyl Proteinases from Microorganisms
微生物新型羧基蛋白酶的结构-功能
  • 批准号:
    08044202
  • 财政年份:
    1996
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
ENVIRONMENTAL DURABILITY OF STRUCTURAL CERAMICS IN HIGH-PRESSURE AND HIGH-TEMPERATURE WATER VAPOR
结构陶瓷在高压高温水蒸气中的环境耐久性
  • 批准号:
    08650998
  • 财政年份:
    1996
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Structure-Function Relationships of Pepstatin-insensitive Carboxyl Proteinases from Prokaryotes
原核生物胃酶抑素不敏感羧基蛋白酶的结构-功能关系
  • 批准号:
    06660105
  • 财政年份:
    1994
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
ENVIRONMENTAL DURABILITY OF SILICON NITRIDE-BORON NITRIDE COMPOSITES
氮化硅-氮化硼复合材料的环境耐久性
  • 批准号:
    06650972
  • 财政年份:
    1994
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Structure-Function Relationships of Pepstation-insensitive Carboxyl Proteinase from Bacteria
细菌胃蛋白酶不敏感的羧基蛋白酶的结构-功能关系
  • 批准号:
    04660125
  • 财政年份:
    1992
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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