Signal substances triggering the activation of vir-region of Ti-plasmid in Agrobacterium tunofaciens and its activation mechanism

根癌农杆菌Ti质粒vir区激活的信号物质及其激活机制

• 批准号: 62560160
• 负责人: TERAZAWA Minoru
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560160/
• 关键词: Agrobacterium tumofaciens; Ti-plasmid; vir-Region; T-DNA; Signal molecule; Wounded tissues; 傷害組織; vir領域活性化物質; アセトシリンゴン; vir領域; 二次代謝; シラカンバ; ポプラ

Signal molecules triggering the activation of vir-region of Ti-plasmid in Agrobacterium tumofaciens and its activation machanizm were investigated.It was confirmed that several low molecular weight phanolic compounds including acetosyringone have ability to activate the vir-rigeion of Ti-plasmid in Agrobacterium tumofaciens.Acetosyringone was not found in the healthy and wounded tobacco plants as free state, glycosides, or depsides. Acetosyringone was not formed from the other phenolic compounds in the wounded tissues. Acetosyringone was detected in the calluses of tobacco plant in this study. Since all the founr plants investigated in this work did not contain acetosyringone in their healthy and wounded tissues, acetosyringone did not seem to be a general signal molecule in plants but to be a characteristic compound in tobacco plant. Each plant may have form the signal molecules characteristic to itself.Structural similarity of the low molecular weight phenolic compounds which can activate the vir-region of Ti-plasmid could give us a hint to understand why the bacterium can catch so many phenolic compounds being belonged to different categoriesas as the signal molecules.

对激活根癌农杆菌钛质粒病毒区的信号分子及其激活机制进行了研究，证实了乙酰丁香酮等几种小分子酚类化合物具有激活根癌农杆菌钛质粒病毒的能力，而在健康和受害的烟草植株中未发现乙酰丁香酮以游离态、糖苷或多苷的形式存在。损伤组织中的其他酚类化合物未形成乙酰丁香酮。本研究在烟草愈伤组织中检测到乙酰丁香酮。由于本工作所研究的所有喷泉植物在其健康和受伤的组织中都不含有乙酰丁香酮，乙酰丁香酮似乎不是植物中的普遍信号分子，而是烟草中特有的化合物。每种植物都可能形成自身特有的信号分子。低分子量酚类化合物能够激活钛质粒的VIR区，其结构上的相似性可以为我们理解细菌为什么能捕捉到如此多属于不同类别的酚类化合物作为信号分子提供线索。

项目成果

Minoru,Terazawa;Hiroto,Mitsugi;Tsutomu,KAYAMA: Mokuzai Gakkaishi. 35. (1989)

寺泽稔；光木宏人；鹿山勉：木哉学会石。

Minoru,Terazawa;Motoi,Chiba;Tsutomu KAYAMA: Mokuzai Gakkaishi. 35. (1989)

寺泽稔；千叶本井；加山勉：木哉学会石。

Minoru TERAZAWA.; Masanori KOBAHASHI.; Tsutomu KAYAMA.: Mokuzai Gakkaishi. 35. (1989)

寺泽稔；

Minoru TERAZAWA;Hiroto MITSUGI;Tsutomu KAYAMA: "Phenolic Compounds in Living Tissues of Woods.X New lignan glycosides in the inner bark of Fagus crenata Blume" Mokuzai Gakkaishi. 35. (1989)

Minoru TERAZAWA;Hiroto MITSUGI;Tsutomu KAYAMA：“木材活组织中的酚类化合物。X 水青冈内皮中的新木脂素糖苷”Mokuzai Gakkaishi。

Minoru,TERAZAWA;Masanori,KOBAYASHI;Tsutomu,KAYAMA: Mokuzai Gakkaishi. 35. (1989)

寺泽稔；小林正则；鹿山勉：木哉学会石。