Study on the function of micro-organisms coexisting in the mass culture of the rotifer Brachionus plicatilis

这是一项基本研究，以使微有机体的功能清晰，其中存在于rotifer Brachionus plicatilis的文化中，是一个用于消除大规模长期退化的生长中仍然存在的大规模生长中的大规模生长中的大规模生长。Results obtained are shown under. 1.作为一个评估在rotifr质量文化中变化的大小频率分布的悬浮固体的结果，动物并没有开始产生细菌块直到平滑的Nannochloropsis，食品手动提供。在那之后，细菌剂的清除率大约是两次，就像Nannochloropsis一样。维生素B12，哪一种已经证明了发酵剂生长的必要条件，它在纳米氯色素瘤或面包师的年份中含有少量的每一个都被普遍用作发酵剂文化的食物。大规模文化中B12的主要来源是为了成为以下结果中细菌的代谢。20%的细菌应变隔离形态轮质培养具有B12生产力，并在其生长阶段生产了1-5 ng/ml·天的B12，在其生长阶段的日志阶段中，与B12需求的B12相当高，并在动物的轴向培养中显示出了可显著的生长促进，而在10^7 - 10^<10>CFU/ml.3。“面包师的营养充足是一个想法，以扩大从B12生产细菌的生长促进的结果，在发酵文化中提供了比面包师的年份更引人注目的纳米氯中毒比在面包师的年份作为一个基本的食品来源。”

This is the fundamental study for making clear the function of micro organisms which are co-existing in the culture of the rotifer Brachionus plicatilis,as a part of researches for dissolving the massmortality of long-term retardation of growth which still occur in mass culture of the animal. Results obtained are shown under.1. As a result of evaluating the changes of size frequency distribution of suspending solids in the rotifr mass culture,the animal did not start to ingest bacterial flocks till swept clear Nannochloropsis,the food manually provided. After then,the clearance rate for bacterial flocks was near twice as much as for Nannochloropsis.2. Vitamin B 12 ,which had been proved to be essential for the growth of the rotifer,is contained little either in Nannochloropsis or baker's yeast which are commonly used as food for rotifer culture. The main source of B 12 in mass culture was thought to be a metabolite of bacteria from the following results. 20 % of bacterial strain isolated form rotifer mass culture had B12 productivity,and produced 1-5 ng/ml・day of B12 in log phase of their growth stages,which is fairly high compared by B12 demand of the rotifer,and showed remarkable growth promotion in the axenic culture of the animal when put in as 10^7 - 10^<10> CFU/ml.3. The nutritive lucking of baker's yeast was thought to extend over a wide range from the results that the growth promotion of B12-producing bacteria was more remarkable in the rotifer culture provided with Nannochloropsis than in that with baker's yeast as a basic food source.