权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study of the marine bacteria that produce plant hormons

产生植物激素的海洋细菌的研究

基本信息

批准号：
62560205
负责人：
SIMIDU Usio
金额：
$ 1.41万
依托单位：
Ocean Research Institute, University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Production of plant hormons, auxin and cytokinin, was tested for bacteria isolated from seawater, sediments, and algae. The proportion of plant hormon-producing strains to total isolates was higer in sediments than in seawater. The percentage of hormon-producing bacteria in seawater was 5-35% for cytokinin, and 20-40% for auxin. The same percentage for sediments was 45-75 and 40-50%, respectively. Bacteria attached to the algal suface also produced hormons in high proportings. This is the first report on the plant hormon production by marine bacteria.The cytokinin was purified from the culture broth of a Vibrio sp., and was identified using gas chromatography-mass spectrometric analysis. Two cytokinins, isopentenyladdenine and isopentenyl-adenosine were identified.Taxonomical examination of hormon-producing bacteria showed that bacteria belonging to many genera as Vibrio, Pseudomonas, Moraxella and Flavobacterium produce hormons.The concentration of plant hormons in seawater and sediments was analyzed using ELISA method. The results showed that marine sediments containe rather high amounts (10^<-6> - 10^<-9>mole/dm^3) of auxin (indole-3-acetic acid.

对从海水、沉积物和藻类中分离出来的细菌的植物激素生长素和细胞分裂素的产生进行了测试。底泥中植物激素产生菌占总菌株的比例高于海水。海水中产生荷尔蒙的细菌中，细胞分裂素的比例为5-35%，生长素的比例为20-40%。沉积物的相同比例分别为45%-75%和40%-50%。附着在藻类表面的细菌也会在高密度时产生荷尔蒙。从一株弧菌的发酵液中分离纯化了细胞分裂素，并用气相色谱-质谱仪对其进行了鉴定。对荷尔蒙产生菌进行了分类鉴定，发现弧菌、假单胞菌、莫拉氏菌和黄杆菌等属的细菌都能产生激素，并用ELISA法分析了海水和沉积物中植物激素的浓度。结果表明，海洋沉积物中含有较高的生长素(吲哚-3-乙酸)(10^&lt；-6&gt；-10^&lt；-9摩尔/dm^3)。