Detection of Early Pregnancy Factor (EPF) in cattle and its application.

目前，EPF的检测主要采用玫瑰花环抑制试验，已有不少研究人员对此进行了研究。对于牛，有几个报告可用，还没有建立测量方法。因此，本研究旨在考察影响牛玫瑰花环抑制试验的各种因素。在花环抑制试验的基础上，对EPF在黄牛妊娠早期诊断中的作用进行了初步探讨。用人工授精后第15天的妊娠血清进行花环抑制试验，测得RIT值平均为6.6~1.29(9~4)，与对照、未妊娠血清的RIT值2.0~0.94(3~0)有显著差异。而孕妇血清RIT值的测定则较好地反映了后来的直肠检查结果，其中9例RIT值大于5的孕妇在孕区产下了新生儿，上述结果表明，人工授精后第15天的孕妇血清RIT值作为早期妊娠的指标是有价值的，EPF在诊断牛早期妊娠中是有效的。

At present EPF is detected with rosette inhibition test and many researchers yary in results. For cattle, a few reports are available and no measuring method has been established yet. Therefore, the present study made in order to examine various factors which influence rosette inhibition test of cattle. On the basis of the results obtained from rosette inhibition test, the effectiveness of EPF as early diagnosis of pregnancy in cattle was also examined.The results of a rosette inhibition test performed according to the modified conditions, using pregnant sera of the 15th day after artificial insemination, revealed measured RIT values of 6.6 1.29 (9-4) on average, with a significance from those of control, non-pregnant sera of 2.0 0.94 (3-0). On the other hand, the measured RIT values of pregnant serum reflected well the results of a rectal examination performed later, and of 9 cases which were at pregnant area of a RIT value of more than 5,7 gave birth ot newborns.The above results suggested both the usefulness of RIT values of pregnant serum of the 15th day after artificial insemination as an index of early pregnancy and the effectiveness of EPF in the diagnosis of bovine early pregnancy.