Detection of Early Pregnancy Factor (EPF) in cattle and its application.

目前，EPF的检测主要采用玫瑰花结抑制试验，许多研究者对其结果尚不清楚.对于牛，只有少数报告可用，尚未建立测量方法。因此，本研究旨在探讨影响牛玫瑰花结抑制试验的各种因素。根据玫瑰花结抑制试验的结果，还对EPF作为早期妊娠诊断的有效性进行了研究，用人工授精后第15天的妊娠血清按改进的条件进行玫瑰花结抑制试验，结果表明，测定的RIT值平均为6.6 ± 1.29（9-4），与对照组相比有显著性差异。未孕血清2.0 ± 0.94（3-0）。另一方面，所测得的孕妇血清的RIT值很好地反映了随后进行的直肠检查的结果，在RIT值大于5的9例孕妇中，结果表明，人工授精后第15天孕妇血清RIT值可作为早期妊娠的指标，EPF在人工授精后早期妊娠中的作用是有效的。奶牛早期妊娠的诊断

At present EPF is detected with rosette inhibition test and many researchers yary in results. For cattle, a few reports are available and no measuring method has been established yet. Therefore, the present study made in order to examine various factors which influence rosette inhibition test of cattle. On the basis of the results obtained from rosette inhibition test, the effectiveness of EPF as early diagnosis of pregnancy in cattle was also examined.The results of a rosette inhibition test performed according to the modified conditions, using pregnant sera of the 15th day after artificial insemination, revealed measured RIT values of 6.6 1.29 (9-4) on average, with a significance from those of control, non-pregnant sera of 2.0 0.94 (3-0). On the other hand, the measured RIT values of pregnant serum reflected well the results of a rectal examination performed later, and of 9 cases which were at pregnant area of a RIT value of more than 5,7 gave birth ot newborns.The above results suggested both the usefulness of RIT values of pregnant serum of the 15th day after artificial insemination as an index of early pregnancy and the effectiveness of EPF in the diagnosis of bovine early pregnancy.