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Cell proliferation kinetics of chronic liver disease.

慢性肝病的细胞增殖动力学。

基本信息

批准号：
62570141
负责人：
SAITOH Kiyoshi
金额：
$ 0.64万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

In chronic liver disease, the histopathological findings of the biopsied tissues, fibrosis of the portal area, inflammatory cell infiltration and piecemeal or focal hepatic cell necrosis are very important. However, they do not always explain the stage of disease. The degree of hepatic injury and fibrosis are changing from time to time on the balance of strength of the causative agents and regenerative capacity of the hepatic cells. In current study, we tried to detect S-phase hepatic cells in the biopsy specimen and compared with histopathological findings to estimate the prognosis.Material and Method: 20 cases of biopsied liver tissues were used for present study. 12 cases were chronic active hepatitis (CAH), 4 were cirrhosis (HC), 2 were chronic inactive hepatitis (CIH), 1 was chronic alcoholic injury (CAI) and 1 was healed acute hepatitis (AH). The liver tissues were incubated a hour in Eagle EME medium containing 3mg/ml Bromodeoxyuridine (BrdU), with 3 atmospheric pressure of 95% … More O_2 and 5% CO_2. After incubation, the tissues were fixed in cold ethanol and embedded in paraffin. Immunohistochemical staining by ABC method was performed using a monoclonal antibody against BrdU (MA-7580, Beckton-Dickinson). The BrdU positive hepatic cells were observed and the positive ratio (labeling index:LI) was calculated.Result and discussion: S-phase hepatic cells were demonstrated in all of the specimens examined, and LI were varied from case to case(1.8-27.3%). The cases of HC (22.4<plus-minus>4.2%), CAH (16.9<plus-minus>3.8%) and AH (27.3%) showed higher LI, and the cases of CIH (3.6<plus-minus>2.6%) and CAI (2.3%) showed low LI. Therefore, the hepatic cells of active chronic liver disease were revealed to regenerate rapidly. The degree of fibrosis was grade as non, slight, moderate, bridging fibrosis and septal formation, and compared with LI. The degree of inflammation and hepatic cell necrosis were graded as non, slight, moderate and marked. As the result, relatively well correlation was observed between LI and degree of fibrosis of the portal area. On the other hand, no significant meanings were noted between LI and inflammatory cell infiltration and hepatic cell nec Less

在慢性肝病中，活检组织的病理表现、门静脉区纤维化、炎症细胞浸润和局部或局部肝细胞坏死是非常重要的。然而，它们并不总能解释疾病的阶段。肝损伤和肝纤维化的程度随病原体强度和肝细胞再生能力的平衡而不断变化。在目前的研究中，我们试图在活检标本中检测s期肝细胞，并与组织病理学结果进行比较，以估计预后。材料与方法：本研究采用20例肝组织活检。慢性活动性肝炎（CAH） 12例，肝硬化（HC） 4例，慢性非活动性肝炎（CIH） 2例，慢性酒精性损伤（CAI） 1例，急性肝炎（AH）痊愈1例。肝组织在含有3mg/ml溴脱氧尿嘧啶（BrdU）的Eagle EME培养基中，在3个大气压95%…More O_2和5% CO_2条件下孵育1小时。孵育后，组织用冷乙醇固定，石蜡包埋。采用抗BrdU单克隆抗体（MA-7580, Beckton-Dickinson）， ABC法进行免疫组化染色。观察BrdU阳性肝细胞，计算其阳性比率（标记指数LI）。结果与讨论：所有检查的标本中均可见s期肝细胞，LI因人而异（1.8-27.3%）。HC（22.4<正负>4.2%）、CAH（16.9<正负>3.8%）和AH（27.3%）患者LI较高，CIH（3.6<正负>2.6%）和CAI（2.3%）患者LI较低。因此，活动性慢性肝病的肝细胞可迅速再生。纤维化程度分为非、轻度、中度、桥性纤维化和间隔形成，并与LI比较。炎症和肝细胞坏死程度分为无、轻度、中度和显著。因此，LI与门静脉区纤维化程度有较好的相关性。另一方面，LI与炎症细胞浸润和肝细胞nec Less之间无显著意义