Study on proliferation and Differentiation of pluripotent hemopoietic stem cells (CFU-S).

本研究由3个独立项目组成，在本财政年度资助期内各项目进展如下：1。多能造血干细胞（CFU-S）与衰老。衰老过程中造血微环境的变化对脾脏菌落形成的影响被特别关注。我们研究了从Dexter的液体培养中收获的CFU-Ss和从受体小鼠的骨髓中重新填充老或年轻骨髓的CFU-Ss。其中一项实验得出结论，老年小鼠的CFU-s对IL-3的责任不低于对照年轻小鼠，将老年或老年供体的骨髓细胞与IL-3一起培养2天，并使培养的细胞形成脾脏菌落。欲了解更多细节，请参考1988年京都日本血液学学会年会摘要，由Y. Hirabayashi等人提交。CFU-S亚组放射敏感性的异质性。这一方案通过一系列的三组实验再次得到证实，并在1988年在中国洪州举行的“大剂量电离和非电离辐射生物效应国际会议”上发表了总结。CFU-S和淋巴祖细胞。为了研究这个项目，与相泽信一博士（RIKEN）合作开发了转基因小鼠，其中所有的造血干细胞都有一个已引入的标记。这将在1989年日本病理学会年会上提出。

Shree independent programs are consisted this study, and each progress during the term of current fiscal year of the grant is as follows: 1. Pluripotent hemopoietic stem cells (CFU-S) and aging. Changes in hemopoietic microenvironment during aging were specifically focussed in terms of the effect on spleen colony formation. We investigated both CFU-Ss that were harvested from Dexter's liquid culture and that from bone marrow of recipient mice been repopulated with an old or a young bone marrow. One of experiments that came to a conclusion showed the responsibility to IL-3, of the CFU-s from old mice was not leser than that of control young mice, when bone marow cells that were harvested from old or yond donor, were cultured with IL-3 for 2 days, and let the cells from the culture form spleen colony. For further details, refer an abstract of the annual meeting of Japanese Hematological Society in Kyoto, 1988, presented by Y. Hirabayashi et al. 2. Heterogeneity of subfractions of CFU-S in radiosensitivity. This program was confirmed again by a series of three set of experiments, and the summary was presented at the International Conference on Biological Effects of Large Dose of Ionizing and Non-ionizing Radiation. 1988 in Hongzhou, China. 3. CFU-S and lymphogenous progenitor cells. In order to study this program, transgenic mice have been developed by a collaboration with Dr. Shin-ichi Aizawa (RIKEN) in which all of the hematopoietic stem cells have a marker that has been introduced. This will be presented at the annual meeting of Japanese Society of Pathology, 1989.