Developmental potency of larval Schistosoma mansoni, and extraction of its mRNA

曼氏血吸虫幼虫的发育效能及其mRNA的提取

• 批准号: 62570180
• 负责人: NOJIMA Hisatake
• 金额: $ 1.47万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570180/
• 关键词: Schistosoma mansoni; Echinostoma paraensei; sporocyst; invertebrate immunity; cercarial productive rhythm; cloning in vivo; developmental potency; hemocyte; 無脊推動物の免疫; セルカリアの産生リズム; セルカリア産出リズム

This studies have been undertaken to mainly know way to match larval development in snails thus capable of obtaining mRNA concerning.A new method for isolation of a large number of sporocysts from intestine of infected snails should have process of digestion by autolysis of intestine removed from infected snails for 12-24 hours at 25 'C.When 0.3 % saline was used for implantation of sporocysts from infected to uninfected snails, snails becoming infected were hardly found (0-0.03 %, positive rate). On contrast, 31.3-55.6 % became infected when Schneider's Drosophila medium was used. These findings indicated that foreigh objects such as medium affected infection rate in implantation of sporocysts. Circulating hemocytes from Echinostoma paraensei-infected M line Biomphalaria snails, or from age and size matched control snails, were studied on plastic slides with phase contrast optics. Fully-spread granulocytes,partially-spread granulocytes, hyalinocytes, round cell were consistently present. Among control snails, the relative percentage and estimated number/mm3 of round cells decline significantly with increased snail size, and the corresponding values for both granulocytes increased. By 8 days post infection, infected snails had significantly higher relative percentage of round cells and partially-spread granulo cytes than control. Phagocytic activity of hemocytes was signi-ficantly lower in infected snails than control. These findings indicated that if susceptibility of snail to parasite was affected by hemocyte population, hemocytes could be used to match larval development in snails.

进行这项研究主要是为了了解如何匹配蜗牛幼虫发育，从而能够获得有关的 mRNA。一种从受感染蜗牛肠道中分离大量孢子被的新方法，应通过从受感染蜗牛中取出的肠道自溶消化过程，在 25°C 下进行 12-24 小时。当使用 0.3% 盐水将受感染蜗牛的孢子囊植入到 未感染的螺，几乎没有发现感染的螺(0-0.03％，阳性率)。相比之下，当使用 Schneider 果蝇培养基时，31.3-55.6% 的人被感染。这些发现表明，培养基等异物影响孢子被植入过程中的感染率。在具有相差光学器件的塑料载玻片上研究了来自副棘毛虫感染的 M 系双脐螺或来自年龄和大小匹配的对照蜗牛的循环血细胞。一致存在完全铺展的粒细胞、部分铺展的粒细胞、透明细胞、圆形细胞。在对照蜗牛中，随着蜗牛尺寸的增加，圆形细胞的相对百分比和估计数量/mm3 显着下降，并且两种粒细胞的相应值都增加。感染后 8 天，受感染的蜗牛的圆形细胞和部分扩散的粒细胞的相对百分比明显高于对照。受感染的蜗牛血细胞的吞噬活性明显低于对照。这些发现表明，如果蜗牛对寄生虫的易感性受到血细胞群体的影响，则可以使用血细胞来匹配蜗牛幼虫的发育。

项目成果

Noda, Shinichi: "Effect of infection with Echinostoma paraensei on circulating hemocyte population of the shail Biomphalaria glabrata" Parasitology.

Noda，Shinichi：“感染paraensei对光滑Biomphalaria glabrata循环血细胞群的影响”寄生虫学。