Analysis of the mechanisms of V_H gene selection in theformation of immunoglovulin heavy chain variable region genes.

V_H基因选择在免疫球蛋白重链可变区基因形成中的机制分析

• 批准号: 62570212
• 负责人: SUGIYAMA Haruo
• 金额: $ 1.47万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570212/
• 关键词: immunoglobulin heavy chain variable region genes; 免疫グロブリン可変部領域遺伝子; 免疫グロブリンH鎖; V_Hgene family

AT 11-2, an Abelson virus-transformed cell line had DJ_H complexes on both chromosomes and is able to form functional variable region genes by the joins of V_H genes to the HJ_H comolexes during culture. Therefore we examined which V_H gene family was used in functional V_H to DJ_H recombinations in ATll-2. Surprisingly, of 32 independent functional V_H to DJ_H recombinational events in ATll-2, 31 events used the V_H segments of the V_H Q52 family, and the remaining one used the V_H segment of the V_H7183 family. Thus, we describe here thefirst B precursor cell line that almost selectively uses the V_HQ52 family in functional V_H to DJ_H rearrangements. The selective use of the V_HQ52 family in this B precursor cell line strongly indicates nonrandom use of V_H genefamilies, and the existence of a stage at which the V_HQ52 family is preferentially used during the normal development of early pre-B cells and has improtatnt implications for understanding the ontogeny of V_H repertoire development. Furthermore, this cell line should prove extremely valuable in further studies of this kind.

在11-2细胞系中，经Abelson病毒转化的细胞在两条染色体上均具有DJ_H复合体，并能在培养过程中通过V_H基因与HJ_H复合体的连接形成功能可变区基因。因此，我们检查了哪个V_H基因家族用于ATll-2中功能性V_H到DJ_H重组。令人惊讶的是，在AT 11 -2中的32个独立的功能性V_H至DJ_H重组事件中，31个事件使用V_H Q52家族的V_H区段，并且剩余的一个使用V_H7183家族的V_H区段。因此，我们在这里描述了第一个B前体细胞系，几乎选择性地使用V_HQ 52家族的功能性V_H到DJ_H重排。V_HQ 52家族在该B前体细胞系中的选择性使用强烈地表明V_H基因家族的非随机使用，并且在早期前B细胞的正常发育期间存在V_HQ 52家族优先使用的阶段，并且对于理解V_H库发育的个体发生具有重要意义。此外，该细胞系在这类进一步研究中应证明是极其有价值的。

项目成果

H.Sugiyama: J.Exp.Med.166. 607-612 (1987)

H.Sugiyama：J.Exp.Med.166。

Y. Tani: "Class switching from 3 to 2b in a murine pre-B cell line." Mol. Immunol.25. 127-136 (1988)

Y. Tani：“小鼠前 B 细胞系从 3 类转换为 2b 类。”

T.Maeda: J.Immunol.138. 2305-2310 (1987)

T.Maeda：J.Immunol.138。

Y.Tani: Mol.Immunol.25. 127-136 (1988)

Y.Tani：Mol.Immunol.25。

Haruo Sugiyama: Journal of Experimental Medicine. 166. 607-612 (1987)

杉山春夫：实验医学杂志。