Analysis of the mechanisms of V_H gene selection in theformation of immunoglovulin heavy chain variable region genes.

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AT 11-2, an Abelson virus-transformed cell line had DJ_H complexes on both chromosomes and is able to form functional variable region genes by the joins of V_H genes to the HJ_H comolexes during culture. Therefore we examined which V_H gene family was used in functional V_H to DJ_H recombinations in ATll-2. Surprisingly, of 32 independent functional V_H to DJ_H recombinational events in ATll-2, 31 events used the V_H segments of the V_H Q52 family, and the remaining one used the V_H segment of the V_H7183 family. Thus, we describe here thefirst B precursor cell line that almost selectively uses the V_HQ52 family in functional V_H to DJ_H rearrangements. The selective use of the V_HQ52 family in this B precursor cell line strongly indicates nonrandom use of V_H genefamilies, and the existence of a stage at which the V_HQ52 family is preferentially used during the normal development of early pre-B cells and has improtatnt implications for understanding the ontogeny of V_H repertoire development. Furthermore, this cell line should prove extremely valuable in further studies of this kind.