Study of the proof for marihuana smoking by gas chromatographic analysis

气相色谱分析证明吸食大麻的研究

• 批准号: 62570265
• 负责人: TAKAHASHI Setsunori
• 金额: $ 1.28万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570265/
• 关键词: Toxicology; Marihuana smoking; ^9-Tetrahydrocannabinol; Capillary GC; ECD; Saliva; キャピラリーGC; ECD; ダ液

For the proof of marihuana smoking, we have tried a new gas chromatographic method for the determination of ^9-tetrahydrocannabinol(THC) in saliva and urine. standard THC was extracted from Cannabis leaves which grown in shimane Prefecture. We developed a gas chromatographic(GC) method with an electron capture defector(ECD) using a reaction pre-column injector for the determination of THC in saliva and urine. One milliliter of the saliva or urine was exrtacted with n-hexane. After washing with each solution of sodium hydroxide and hydrochloric acid, the n-hexane layer was evaporated under nitrogen gas. The residue dissolved in ethyl acetate and THC containing residue was pentafluoropropionic(PFP) derivatives. p,p'-DDT was used as internal standard. The GC/ECD analysis of THC/PFP had a sensitivity of 1 ng/ml of saliva and good linearity was observed on calibration curve in concentration range of 5 to 200 ng/ml. Recoveries of THC added to saliva and urine were 84 % and 64 %, respectively. We have examined experimental smoking for detecting in the saliva and urine of men who smoked a single merihuana cigarette that contained 0.5 g of cannabis leaves equivalent to 10 mg of THC. After smoking model cigarette, the THC levels in saliva were 58.3-250.0 ng/ml at l h, 79.5-156.3 ng/ml at 2 h, and declined at 34.1-74.1 ng/ml 4 h after. On the other hand the urinary THC levels were lower and trace amounts even if the THC can be detectable. In conclusion, the capillary GC/ECD analysis of THC in saliva would be useful for the detection of marihuana abusers.

为了证明吸食大麻，我们尝试了一种新的气相色谱法测定唾液和尿液中的四氢大麻酚（THC）。标准四氢大麻酚是从生长在岛根县的大麻叶中提取的。本文建立了一种气相色谱（GC）检测唾液和尿液中四氢大麻酚（THC）的方法。用正己烷萃取一毫升唾液或尿液。用氢氧化钠和盐酸溶液洗涤后，在氮气下蒸发正己烷层。溶解在乙酸乙酯中的残渣和含四氢大麻酚的残渣为五氟丙酸衍生物。p,p'-DDT作为内标。THC/PFP的GC/ECD分析灵敏度为1 ng/ml唾液，在5 ~ 200 ng/ml浓度范围内与校准曲线呈良好的线性关系。唾液和尿液中添加四氢大麻酚的回收率分别为84%和64%。我们对抽一支含有0.5克大麻叶相当于10毫克四氢大麻酚的大麻烟的男性进行了唾液和尿液检测。吸模型烟后，1 h唾液中THC含量为58.3 ~ 250.0 ng/ml， 2 h为79.5 ~ 156.3 ng/ml， 4 h后下降为34.1 ~ 74.1 ng/ml。另一方面，尿中四氢大麻酚水平较低，即使四氢大麻酚可以检测到，也是微量的。综上所述，毛细管气相色谱/ECD分析唾液中四氢大麻酚可用于大麻滥用者的检测。

项目成果

Maseda C.,et al.: Forensic Science International. 32. 259-266 (1986)

Maseda C. 等人：国际法医科学。