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Localization of acetylcholine receptor in Hirschsprung's disease

先天性巨结肠中乙酰胆碱受体的定位

基本信息

批准号：
62570614
负责人：
ITO Yasuo
金额：
$ 1.6万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570614/
关键词：
Hirschsprung's disease Megacolon Acetylcholine receptor Autoradiography オートラジオグラフィー

项目摘要

It is not clearly defined why the aganglionic colon in Hirschsprung's disease remains contracted. Our electronmicroscopic study on acetylcholinesterase activity in Hirschsprung's disease revealed localization of the enzyme in the interspace between extrinsic nerve terminals and smooth muscle cells.Our research was aimed to clarify if the acetylcholine receptors exist on the smooth muscle cells to receive innervation of the extrinsic nerve fibers.Method: Localization of muscarinic acetylcholine receptors in the intestine of normal rats and Hirschsprung's rats was studied by autoradiography using [3H] quinuclidinyl benzilate.Results: Silver grains representing acetylcholine receptors were shown to distribute diffusely in the mucosa and submucosa, and linearly on the surface of muscular layers in normal rats. The grains were not recognized in the atropine-treated rats, indicating specificity of the grains to muscarinic acetylcholine receptors. Muscarinic acetycholine receptors were demonstrated also in the aganglionic colon of Hirschsprung's rats. The density of silver grains in the aganglionic colon did not differ much from that in the normal colon.Conclusions: Muscarinic acetylcholine receptors were present in the aganglionic colon of Hirschsprung's rat as in the normal rat colon. This finding indicates that smooth muscles of the aganglionic colon of Hirschsprung's disease may be directly innervated by the proliferated extrinsic nerves.

为什么先天性巨结肠的无神经节结肠仍然收缩，目前还不清楚。我们对先天性巨结肠的乙酰胆碱酯酶活性进行了电镜研究，发现乙酰胆碱酯酶主要定位于外源性神经末梢与平滑肌细胞之间的间隙，目的是为了阐明平滑肌细胞上是否存在接受外源性神经纤维支配的乙酰胆碱受体。用[~ 3 H]奎宁环二苯乙酸酯放射自显影法研究了正常大鼠和先天性巨结肠大鼠小肠中毒蕈碱型乙酰胆碱受体的定位。在正常大鼠胃粘膜和粘膜下层呈弥漫性分布，在肌层表面呈线状分布，代表乙酰胆碱受体的银颗粒。在阿托品处理的大鼠中未识别出颗粒，表明颗粒对毒蕈碱乙酰胆碱受体具有特异性。毒蕈碱型乙酰胆碱受体也被证明在无神经节结肠的先天性巨结肠。无神经节结肠中的银颗粒密度与正常结肠中的银颗粒密度差异不大。结论：与正常大鼠结肠一样，无神经节结肠中也存在M受体。这一发现表明，平滑肌的无神经节结肠的先天性巨结肠病可能是直接支配的增生的外源性神经。