HISTOPATHOLOGICAL CHARACTERISTICS OF THE EARLY STAGE AND ONCOGENOUS EXPRESSION IN PRIMARY HEPATOCELLULAR CARTINOMA

原发性肝细胞癌早期的组织病理学特征及癌性表达

• 批准号: 62570624
• 负责人: OKAMOTO Eizo
• 金额: $ 1.6万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570624/
• 关键词: Early hepatocellular carcinoma; Cytomorphological characteristics; Cytometrical analysis; Immunohistochemistory; DNA ploidy; Onchogenous expression; 免疫組織化学; フローサイトメトリー; 核DNA解析; 細小肝癌; 微小肝癌の形態; 肝線腫; 肝硬変; α-フェトプロテイン

Early diagnosis - treatment is essential for improving the prognosis of hepatocellular carcinoma (HCC) However, the Pathomorphology of this disease in the early stage has hardly be elucidated.In the totally 472 resected cases of HCC, 111 cases of small liver cancer(less than 3cm in diameter) containing of 23 cases of minute liver cancer(less than 2 cm in diameter) were investigated by cytomorphological analysis, immunohistochemical study, flow cytometric study on nuclear DNA analysis and expression of cellular oncogenes.1)In the histology of non-cancerous region not only completed cirrhosis of type B but also a precirrhotic lesion from chronic hepatitis to type B'cirrhosis were observed in a considerable number, especially in large liver cancers.2)Minute liver cancers showed extracapsular invasion in 60%, intrahepatic metasis in 25%. Considerable advance was observed even when the tumor was less than 2cm in diameter. The HCC with slight or mild cirrhosis revealed the low rates of invas … More ion and metastasis.3)Small liver cancer consisted primarily of differentiated cells of Ed I and II types.4)In the distant areas from main tumors, minute daughter masses with no capsule and poorly defined border, consisting smaller, proliferative hepatocellular-cells were observed, and this lesions have been frequently called adenomatous hyperplasia. However, this minute mass showed high N/C ratio in the cytometry, and often had small foci of HCC, though showing negative tumor makers in the immunohistochemistry. Therefore we consider this minute focus to be, indeed, the early lesion of UCC.5)In the DNA ploidy, small liver cancers showed a significantly higher rate of diploid pattern compared with large tumors.6)In the oncogenous expression, 5 oncogenes of C-myc, C-Ha-ras, N-ras. lca and C- fos were examined in 17 HCC, and N-ras and C-myc were elevated in nearly all cases. However no oncogenous expression were detected in the minute cancers.7)The morphology of a early stage of HCC was suggested to be highly well-differentiated cytomorphologic features that show few deviations from normal liver cells. Less

早期诊断-治疗对于改善肝细胞癌（HCC）的预后至关重要。然而，该病早期的病理形态学尚不清楚。在472例切除的HCC病例中，通过细胞形态学分析，对111例小肝癌（直径小于3cm）和23例微小肝癌（直径小于2cm）进行了分析， 免疫组化、流式细胞术研究核DNA分析及细胞癌基因表达。1）非癌区组织学上不仅有完全B型肝硬化，而且有相当数量的从慢性肝炎到B'型肝硬化的肝硬化前病变，尤其是大肝癌。2）微小肝癌60％出现包膜外侵犯， 肝内转移25%。即使肿瘤直径小于2厘米，也能观察到相当大的进展。轻度或轻度肝硬化的 HCC 侵袭和转移率较低。3）小肝癌主要由 Ed I 和 II 型分化细胞组成。4）在距主肿瘤较远的区域，观察到无包膜、边界不清的微小子肿块，由较小的增殖性肝细胞组成，这种病变通常称为腺瘤性增生。然而，这种微小的肿块在细胞计数中显示出较高的 N/C 比，并且通常具有较小的 HCC 病灶，尽管在免疫组织化学中显示肿瘤标志物呈阴性。因此，我们认为这个微小的焦点确实是UCC的早期病变。5）在DNA倍体方面，与大肿瘤相比，小肝癌表现出明显更高的二倍体模式。6）在癌基因表达方面，C-myc、C-Ha-ras、N-ras 5个癌基因。在 17 例 HCC 中检查了 lca 和 C-fos，几乎所有病例中 N-ras 和 C-myc 均升高。然而，在微小的癌症中没有检测到癌源性表达。7)早期HCC的形态被认为是高度分化的细胞形态学特征，与正常肝细胞几乎没有偏差。较少的

项目成果

藤元治朗: "肝細胞癌の核DNA解析" 日本外科学会雑誌. 90. 1568-1570 (1989)

Jiro Fujimoto：“肝细胞癌的核 DNA 分析”日本外科学会杂志 90. 1568-1570 (1989)。

豊坂昭弘: "肝細胞癌の前癌病変及び境界領域病変の臨床病理学的研究ー小肝癌肝切除例における検討ー" 第26回日本癌治療学会総会(新潟). (1988)

Akihiro Toyosaka：“肝细胞癌癌前和边缘病变的临床病理学研究 - 小肝癌肝切除术病例的检查 -”第 26 届日本癌症治疗学会年会（新泻）（1988 年）。

Toshihiro Kato, Eizo Okamoto, Akihiro Toyosaka, Naoki Yamanaka, Masao Mitsunobu and Jiro Fujimoto: "Long-term results of small sized hepatocellular carcinoma after hepatectomy" Journal of Hyogo Association of Surgeons, 94, 41-46, 1988.

Toshihiro Kato、Eizo Okamoto、Akihiro Toyosaka、Naoki Yamanaka、Masao Mitsunobu 和 Jiro Fujimoto：“肝切除术后小型肝细胞癌的长期结果”兵库外科医师协会杂志，94, 41-46, 1988。

Okamoto Eizo: "Current status of Hepatic resection in the treatment of Hepatocellular Carcinoma" Neoploms of the liver Editors K.Okuda,K.Gishak. 353-365 (1987)

冈本英三：“肝细胞癌治疗中肝切除术的现状”Neoploms of the Liver 编辑 K.Okuda、K.Gishak。

光信正夫: "肝細胞癌の前癌病変及び類似病変の臨床病理学的研究" 第33回日本消化器外科学会総会(東京). (1989)

Masao Mitsunobu：“肝细胞癌癌前病变和类似病变的临床病理学研究”第33届日本胃肠外科学会年会（东京）（1989年）。