Clinical and experimental studies on early diagnosis and improvement of therapeutic effect pancreatic cancer using monoclonal antibodies.

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Pancreatic carcinoma is associated with a poor prognosis, and the results of treatment are still disappointing. In an effort to improve the prognosis, the monoclonal antibodies (MoAb) reactive with pancreatic carcinoma were established and the serological markers for early diagnosis and the targeting chemotherapy for the disease were evaluated using these MoAb.In a practical application of drug delivery system, anticancer drug conjugated with dextran (ADM-OXD) was demonstrated to be effective for human pancreatic cancer cell lines. Studies on the pharmaceutical characteristics of the ADM-OXD were performed and the phase 0 study have been started.The new immunohistochemical assay, modified direct immunoperoxidase method for detection of human MoAb reactive with gastrointestinal carcinoma was established. A human MoAb derived from spleen cell of a gastric cancer patient was demonstrated to react with adenocarcinoma of stomach, colon and pancreas in the new direct immunohistochemical assay. The possible use of human MoAb for clinical application of targeting cancer chemotherapy was suggested to be possible in the future.The sensitivity and specificity of chimeric antibodies of mouse MoAb such as KM10 and A10 with human IgG were evaluated and the possible use of the chimeric antibodies for clinical application was found by means of large scale of culures.