Development of Chemical Functions of Enzymes

“酶的化学功能开发”研究项目从酶和辅酶模型系统的构建和应用、高效分子识别位点的设计、重要生物金属配合物的合成和应用、生物系统调节化合物的开发等方面展开。本文的研究结果总结如下：1)建立了藜蛋白和黄酮类蛋白的高效模型体系，并尝试将其化学功能应用于有机合成。2)利用NAD(P)H模型阐明了生物及其模拟反应体系中立体化学的区分机理。3)用几种含有咪唑配体的表面活性剂合成了锌酶模型配合物，并考察了它们在酯水解中的催化作用。4)功能化双层膜已被证明是一种高效的酶模型系统。5)通过微溶剂化的“非选择性”底物结合首次被证实。6)利用合成双层膜实现了酶和酶模型的选择性结合。7)合成了几种功能化卟啉配体，并对其分子识别能力等化学功能进行了研究。8)血红素酶模拟催化剂是一种可用于合成化学的高效氧合体系。9)利用锌配合物与多胺配体设计分子识别位点来阐明生物金属功能。10)合成了钼氧化酶模型配合物，并对其结构和性质进行了研究。阐明了配位二氮在钼、钨配合物中的反应活性。铜和铁的mu-过氧配合物已被制备，它们的反应活性已作为携氧酶和单加氧酶的模型进行了研究。为了整理结果，1991年2月5日在大阪召开了一次会议。从两年来的许多突出发现和突破来看，认为每位研究者的成果都达到了本项目的目标。少

The research project on "Development of Chemical Functions of Enzymes" has been carried out from the viewpoints of i) construction and application of enzyme and coenzyme model systems, ii) design of highly efficient molecular recognition sites, iii) synthesis and application of biologically important metal complexes, exes, and iv) development of regulatory compounds in biological systems.The summary of the results of the present research is as follows ; 1) Efficient model systems of quinoproteins and flavoproteins have been established and their chemical functions have been attempted to apply to organic synthesis. 2) Mechanism to differentiate stereochemistry in biological and its mimetic reaction systems has been clarified by using NAD(P)H models. 3) Model complexes of zinc enzymes have been synthesized by using several surfactants containing imidazole ligands, and their catalytic functions have been examined in ester hybrolysis. 4) Functionalized bilayer membrane has been demonstrate … More d to provide an efficient enzyme model systems. 5) "Nonselective" substrate binding via microsolvation has been first demonstrated. 6) Selective binding of enzymes and enzyme models has been achieved by using synthetic bilayer membranes. 7) Several types of functionalized porphyrin ligands have been synthesized and their chemical functions including molecular recognition ability have been investigated. 8) Heme enzyme mimetic catalyst has been developed to construct a highly efficient oxygenation systems which can be applicable to synthetic chemistry. 9) Molecular recognition sites have been designed by using zinc complexes with polyamine ligands to elucidate biometal functions. 10) Molybdo-oxidase model complexes have been synthesized and their structure and properties have been investigated. 11) Reactivities of Coordinated dinitrogen in molybdenum and tungsten complexes have ben clarified. 12) mu-Peroxo complexes of copper and iron have been prepared and their reactivities have been examined as models for oxygen carries and monooxygenases.In order to organize the results, a meeting was held on February 5, 1991 at Osaka. It is considered that the results of each investigators have attained goal of this project judging from many outstanding findings and breakthroughs during the past two years. Less