Development of an Near-infrared and Visible Time-resolved Spectrophotometer System

近红外和可见光时间分辨分光光度计系统的研制

• 批准号: 01840016
• 负责人: SHIZUKA Haruo
• 金额: $ 3.97万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01840016/
• 关键词: Near-infrared emission spectrum; Near-infrared absorption spectrum; Transient absorption spectrum; Germanium photodiode detector; Time resolved spectrofluorimeter; Lifetime measurement

The purpose of this research is to construct a convenient spectrophotometer system which is able to measure the absorption and emission spectra of unstable intermediates such as radicals and triplet states in near-infrared region. Because of lack of a good detector in this region, it has been difficult to measure them quantitatively and therefore the spectroscopic study in near-infrared region has stayed in an undeveloped stage.In this research, we tried to construct a spectrophotometer system which is able to measure absorption spectro and emission specctra in visible and near-infrared region, in combination with a Nd^<3+> : YAG laser system. A photomultiplier and a germanium diode were used as the detectors in visible and near infrared region, respectively. By studying the spectra of some laser dyes, it was confirmed that the expected data were obtained by the constructed spectrophotometer.The ability of our system is as follows ;(1) Spectral wavelength region is from visible to near infrared (400nm-1800nm).(2) Time resolution is -20ns.(3) The spectrophotometer system is controlled and the experimental data are processed by a microcomputer.By use of our near-infrared spectrophotometer system, absorption and fluorescence spectra, and fluorescence lifetimes of semiquinone radicals of xanthone, benzoylnaphthone, dibenzilidene acetone and flavone produced in poly (vinyl alcohol) films by gamma-irraidation. Fist several absorption bands and therefore the fluorescence bands of these radicals are in near-infrared region. The long wavelength location is the reason why the fluorescence spectra of these radicals have not been measured until very recently.We succeeded in measuring the fluorescence spectra of these radicals for the first time. It was also found that the fluorescence lifetimes of these radicals are shorter than the pulse width of laser excitation laser (8ns).

本研究的目的是构建一种方便的分光光度计系统，能够测量自由基和三重态等不稳定中间体在近红外区域的吸收和发射光谱。由于该区域缺乏良好的探测器，很难对其进行定量测量，因此近红外区域的光谱研究一直处于不发达的阶段。在本研究中，我们试图结合Nd^3+:YAG激光系统构建一个能够测量可见光和近红外区域吸收光谱和发射光谱的分光光度计系统。光电倍增管和锗二极管分别用作可见光和近红外区域的探测器。通过研究一些激光染料的光谱，证实所构建的分光光度计得到了预期的数据。我们的系统的能力如下：（1）光谱波长范围从可见光到近红外（400nm-1800nm）。（2）时间分辨率为-20ns。（3）分光光度计系统由微机控制，实验数据处理。 我们的近红外分光光度计系统的吸收和荧光光谱，以及通过伽马辐照在聚（乙烯醇）薄膜中产生的呫吨酮、苯甲酰萘酮、二苯亚甲基丙酮和黄酮的半醌自由基的荧光寿命。首先有几个吸收带，因此这些自由基的荧光带位于近红外区域。长波长位置是这些自由基的荧光光谱直到最近才被测量的原因。我们首次成功地测量了这些自由基的荧光光谱。还发现这些自由基的荧光寿命比激光激发激光的脉冲宽度（8ns）短。