Simulation Test of Hemolysis by Micro-dye-Capsule Solution in a Centrifugal Blood Pump.

血液的溶血、凝血以及泵的动力性能是血泵研究中的重要问题。研制叶轮高速旋转时溶血率低的离心血泵显得尤为重要。由于材料表面的生物反应，用真血进行高重复性的溶血试验是不容易的。因此，如果我们使用对动力敏感但生物惰性的微染料胶囊代替红细胞，那么我们可以期望仅由纯动力效应引起的高度可重复的溶血数据。这些胶囊是工业用压力传感片的颗粒。测试结果如下：“1”。我们建立了用微染料胶囊溶液（富士胶片公司生产）溶血的模拟试验方法。受损胶囊中的无色染料在溶液中以液滴的形式游离。在颜色感应片上测量染料浓度表明胶囊的损坏等级。这与真正溶血试验中的游离血红蛋白非常相似。“2”。双锥型和旋动式离心血泵的溶血试验结果表明，胶囊的损伤可以用剪切应力乘以时间的指数函数来表示。当转速超过3500转/分时，胶囊损伤严重。我们的旋转离心泵的溶血阈值被证明是3500转/分。这与真血溶血试验结果吻合较好。

Hemolysis and coagulation of blood as well as dynamical pump performance are serious problems in blood pumps. It is specially important to develop a centrifugal blood pump with low hemolysis even under high speed rotation of impeller. Highly repeatable hemolysis test by real blood is not easy owing to biological surface reaction on materials. Therefore, if we, as substitute of red blood cell, use micro-dye-capsules, which are sensitive to dynamical forces but biologically inert, then we can expect highly repeatable hemolysis data only caused by pure dynamical effects. These capsules are particles for pressure sensing sheets of industrial use.The test results are as follows : "1". We established simulation test methods of hemolysis with micro-dye -capsule solutions (produced by Fuji Film Inc.). The leuco dye in the damaged capsule becomes free as droplets in the solutions. Measured dye concentration on a color sensing sheet indicates thedamaged grade of the capsules. This is very similar to free hemoglobin in real hemolysis test. "2". Hemolysis tests of in double cones and nutating centrifugal blood pumps result that the damage of capsules is expressible with an exponential function of shear stress multiplied by elapse time. The damage of capsules increase severely at the higher rotational speed than 3500 rpm. The threshold of hemolysis for our nutating centrifugal pump proved to be 3500 rpm. This correlate well with hemolysis test by real blood.