Analysis of the growth mechanisms of a species causing freshwater red tide in a reservoir using in situ microcosms

利用原位微观世界分析引起水库淡水赤潮的物种的生长机制

• 批准号: 63540521
• 负责人: KAWABATA Zen'ichiro
• 金额: $ 1.15万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540521/
• 关键词: reservoir; freshwater red tide; dinoflagellate; Ceratium; cyst; microcosm; depth; soluble organic phosphorus; 渦鞭毛藻; 発芽率; 内部負荷; 流入河川水

The dinoflagellate Ceratium hirundinella (O. F. Muller) Bergh often causes freshwater red tide in reservoirs. There has been, however, little investigation on the growth mechanisms of C. hirundinella in a reservoir due to the difficulty in obtaining its physiological characteristics from laboratory cultures.The purpose of this study was to find the factors for the excystment and growth of C. hirundinella in a reservoir by culture experiments using microcosms in situ. In addition, field surveys and laboratory experiments were also carried out. The main results obtained were as follows:1. Microcosms containing bottom mud with cysts of C. hirundinella were suspended at several depths when the water temperature was 20 ﾟC at all depths. More vegetative cells were found in the shallower depths and the excystment rate was 2 % at 0.5 m deep. No vegetative cells were found deeper than 30 m.2. From the laboratory culture of the cysts, the optimum water temperature for excystment was 20 ﾟC and 25 ﾟC, here the excystment rate was 2 %. No excystment was observed lower than 10 ﾟC.3. The period of excystment was predicted to be from May to September and the place for excystment to be the head of the reservoir, according to results 1 and 2 mentioned above.4. It was suggested from a field survey that soluble organic phosphorus favors the growth of C. hirundinella.5. Microcosms with PO_4-P and NO_3-N added contained higher concentrations of soluble organic phosphorus and C. hirundinella compared with microcosms without the addition of those nutrients. The microcosm experiment also suggested that soluble organic phosphorus was a factor favoring the growth of C. hirundinella.

甲藻Ceratium hirundinella（O. F. Muller）Bergh经常引起水库的淡水赤潮。然而，关于C.由于实验室培养难以获得其生理特性，导致水库中的hirundinella数量减少，本研究的目的是寻找影响hirundinella脱囊生长的因素。通过微宇宙原位培养试验，研究了水库中的Hirundinella此外，还进行了实地调查和实验室试验。主要研究结果如下：1.微宇宙含有底泥和C.当水温为20 ℃时，hirundinella悬浮在几个深度。营养细胞在较浅的深度发现较多，在0.5m深度脱囊率为2%。没有营养细胞被发现深度超过30米。从实验室培养的孢囊来看，20 ℃和25 ℃是脱囊的最适水温，此时脱囊率为2%。在低于10 μ C时未观察到脱囊。根据上述结果1和2，预测了水库的脱囊期为5 ~ 9月，脱囊地点为库首.田间调查结果表明，可溶性有机磷有利于青冈的生长。hirundinella 5.添加PO_4-P和NO_3-N的微宇宙中可溶性有机磷和C的含量较高。与没有添加这些营养素的微宇宙相比，微宇宙实验还表明，可溶性有机磷是一个有利于C.水蛭属

项目成果

Kawabata, Z.: "Effect of water temperature on the excystment of the dinoflagellate Ceratium hirundinella (O.F.Muller) Bergh"

Kawabata, Z.：“水温对甲藻 Ceratium hirundinella (O.F.Muller) Bergh 排泄的影响”

Kawabata,Z.: "Changes in phytoplankton biomass and water quality in microcosms placed in a reservoir"

Kawabata,Z.：“水库微观世界中浮游植物生物量和水质的变化”

Kawabata,Z.: "Distribution pattern of the dinoflagellate Ceratiumhirundinella(O.F.Muller)Bergh in a rservoir" Hydrobiologia. 169. 319-325 (1988)

Kawabata，Z.：“水库中甲藻 Ceratiumhirundinella (O.F.Muller)Bergh 的分布模式”水生生物学。

Kawabata,Z.: "Conditions and sites of excystment for the dinoflagellate Ceratium hirundinella Ceratium hirundinella(O.F.Muller)Bergh in a reservoir"

Kawabata,Z.：“水库中甲藻 Ceratium hirundinella Ceratium hirundinella(O.F.Muller)Bergh 的排泄条件和位点”

Kawabata, Z.: "Conditions and sites of excystment for the dinoflagellate Ceratium hirundinella (O.F.Muller) Bergh in a reservoir"

Kawabata, Z.：“水库中甲藻 Ceratium hirundinella (O.F.Muller) Bergh 的排泄条件和位点”