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Research for Growth Factor Receptors in Human Tumor Tissues

人类肿瘤组织中生长因子受体的研究

基本信息

批准号：
63570145
负责人：
SHIGEMATSU Kazuto
金额：
$ 1.34万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570145/
关键词：
Insulin-Like Growth Factor I Epidermal Growth Factor Receptor Brain Tumor Lung Cancer Adrenal Tumor Receptor Autoradiography Immunohistochemistry インシュリン成長因子 IGF-I 定量的receptor autoradigraphy ラット脳ヒト副腎

项目摘要

Receptors of insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) were analyzed in normal and tumor tissues.1) Brain tumors: Receptors of IGF-I were widely localized in the rat brain areas regulating the behavior, central blood pressure and pituitary hormone. Taken together with the expression of IGF-I mRNA in the brain, the possibility that IGF-I plays the role of a neurotransmitter and/or neuromodulator in the central nervous system is considered. High number of IGF-I and EGF receptors were homogeneously present in tissue sections drived from meningioma, glioblastoma and anaplastic ependymoma, whereas these receptors were faintly present in normal cortex. IGF-I receptors, but not EGF receptors, were also observed in medulloblastoma. Both growth factors stimulated the synthesis of DNA in a dose-dependent manner in the culture of tumor cells. These observations can be interpreted to mean that both IGF-I and EGF may be involved in the growth modulation of brain tumors.2) Lung tumors: We found that IGF-I receptors were present on squamous cell carcinoma, adenocarcinoma, small cell carcinoma and large cell carcinoma. High number of IGF-I receptors were present in cases of squamous cell carcinoma and small cell carcinoma, while cases of adenocarcinoma showed low number. High number of IGF-I receptors, without immunoreactivity, were observed in cases of small cell carcinoma.3) Adrenal tumors: Both IGF-I and EGF receptors were located in the adrenal cortex, while the adrenal medulla contained only IGF-I receptors. However, we could not find the expression of IGF-I receptors in the adrenal tumors. The frequency of EGF receptors expression in the adrenocortical carcinomas (98.4%) was significantly higher than in the adrenocortical adenomas (43.5%) and pheochromocytomas (57.1%). We conclude that the expression of EGF receptors is associated with tumor growth and/or metastatic potential in adrenocortical carcinomas.

对正常组织和肿瘤组织中的胰岛素样生长因子 I (IGF-I) 和表皮生长因子 (EGF) 受体进行了分析。 1) 脑肿瘤：IGF-I 受体广泛定位于调节行为、中枢血压和垂体激素的大鼠大脑区域。结合大脑中IGF-I mRNA的表达，考虑IGF-I在中枢神经系统中发挥神经递质和/或神经调节剂作用的可能性。大量 IGF-I 和 EGF 受体均匀存在于脑膜瘤、胶质母细胞瘤和间变性室管膜瘤的组织切片中，而这些受体微弱地存在于正常皮质中。在髓母细胞瘤中也观察到 IGF-I 受体，但未观察到 EGF 受体。在肿瘤细胞培养物中，两种生长因子均以剂量依赖性方式刺激DNA的合成。这些观察结果可以解释为IGF-I和EGF可能参与脑肿瘤的生长调节。2)肺癌：我们发现IGF-I受体存在于鳞状细胞癌、腺癌、小细胞癌和大细胞癌上。鳞状细胞癌和小细胞癌病例中存在大量 IGF-I 受体，而腺癌病例中 IGF-I 受体数量较少。小细胞癌中观察到大量无免疫反应性的IGF-I受体。3)肾上腺肿瘤：IGF-I和EGF受体均位于肾上腺皮质，而肾上腺髓质仅含有IGF-I受体。然而，我们在肾上腺肿瘤中未发现IGF-I受体的表达。肾上腺皮质癌中EGF受体表达频率(98.4%)显着高于肾上腺皮质腺瘤(43.5%)和嗜铬细胞瘤(57.1%)。我们得出的结论是，EGF 受体的表达与肾上腺皮质癌的肿瘤生长和/或转移潜力相关。