权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The Role of Maternal Antibody and Complement in Protection of Young Mice Against Infection with Trypanosoma Gambiense.

母体抗体和补体在保护幼鼠免受冈比亚锥虫感染中的作用。

基本信息

批准号：
63570179
负责人：
TAKAYANAGI Tan
金额：
$ 1.34万
依托单位：
Medical School, Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

There are at least two routes of antibody transmittance from hyperimmune females into fetal circulation. The one is placental and yolk sac route during pregnancy, the other is colostral route after birth. Antibodies which move through the placenta or yolk sac or colostral route are IgG molecules. By suckling colostra from hyperimmune mother, agglutinin activity in young mouse serum increased rapidly. Young mice with colostral IgG antibody showed protection against trypanosomes. So, colostral IgG antibody is the main component which is responsible for clearance of pathogens. I try to inquire further into the mechanism by which host immune protection is facilitated. Aggregated trypanosome masses formed in equivalence area without complement are readily dissociated by complement.Complement-mediated dissociation of the clumped trypanosomes in the equivalence area released approximately fifty percent of previous bound surface antigens. These antigens are capable of binding again to new IgG antibody. Experiments indicate that complement deposition alters bivalent IgG antibody in the immune complex into a univalent one. Such event in the presence of complement is of great advantage to the infected host in killing pathogens in vivo, as it allows more antibodies to attach to surface antigens and subsequently initiate complement activity. Complement can fulfill the dissociating function whether it is activated by the classical or by the alternative pathway.

至少有两种途径抗体从高免疫雌性进入胎儿循环。一种是妊娠期经胎盘和卵黄囊途径，另一种是产后经初乳途径。通过胎盘或卵黄囊或初乳途径的抗体是IgG分子。哺乳高免疫母鼠的初乳，幼鼠血清凝集素活性迅速升高。具有初乳IgG抗体的年轻小鼠显示出针对锥虫的保护作用。因此，初乳IgG抗体是负责清除病原体的主要成分。我试图进一步探究宿主免疫保护的机制。在没有补体的等效区中形成的聚集锥虫块很容易被补体解离。在等效区中聚集锥虫的补体介导的解离释放了大约50%的先前结合的表面抗原。这些抗原能够再次结合新的IgG抗体。实验表明，补体沉积将免疫复合物中的二价IgG抗体改变为单价IgG抗体。在补体存在下的这种事件对于感染的宿主在体内杀死病原体是非常有利的，因为它允许更多的抗体附着于表面抗原并随后启动补体活性。补体无论是通过经典途径还是旁路途径激活，都可以完成解离功能。