Studies on the Protein Synthesis by Toxoplasma Gondii in Cell Free System.

无细胞系统中弓形虫蛋白质合成的研究。

基本信息

  • 批准号:
    63570186
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1988
  • 资助国家:
    日本
  • 起止时间:
    1988 至 1990
  • 项目状态:
    已结题

项目摘要

The main reason for studying Toxoplasma is to learn more about its behavior in hopes of subsequently discovering a method for controlling or eradicating the disease it causes. Despite intensive study by experimental infection of various animals and tissue cultures, relatively little is known about this parasite's requirements for infection, survival and growth in a host cell. Nutritional studies of this parasite are complicate by its obligate parasitic nature. They are hidden from view of most of their life cycle. The extra-host cellular stage of the parasite, namely tachyzoite, which is easily obtainable in quantity seemed to be inactive or depressed, if any, in its metabolic activity. We examined influences of extracellular ions on several cellular events of this parasite including regulation of internal pH, motility and infectivity. We also studied possible occurrence of incorporation of ^<35>S-methionine by the tachyzoites in cell-free medium with varying external ionic conditions. In the present report, external ionic conditions, mainly K^+ and C1^-, are discussed in terms of electrochemical H^+ gradients across the parasite's membrane for cellular events of the tachyzoite in cell-free medium. A successful application of metabolic radiolabelling of toxoplasma proteins by ^<35>S-methionine let us possible tracer experiments of this parasitic protozoon. This method may also useful for screening anti-toxoplasma agints.
研究弓形虫的主要原因是更多地了解其行为,希望随后发现控制或根除其引起的疾病的方法。尽管通过各种动物和组织培养的实验感染进行了深入的研究,但对这种寄生虫在宿主细胞中感染、存活和生长的要求知之甚少。这种寄生虫的营养研究因其专性寄生性质而复杂化。它们在其生命周期的大部分时间里都是隐藏的。寄生虫的宿主细胞外阶段,即速殖子,很容易大量获得,但其代谢活动似乎不活跃或受到抑制(如果有的话)。我们研究了细胞外离子对这种寄生虫的几个细胞事件的影响,包括调节内部pH值,运动性和感染性。我们还研究了<35>在不同外部离子条件下,速殖子在无细胞培养基中掺入^S-甲硫氨酸的可能性。在本报告中,外部离子条件,主要是K^+和Cl ^-,讨论了在无细胞培养基中速殖子细胞事件中,寄生虫细胞膜上的电化学H^+梯度。用~(135)S-蛋氨酸标记弓形虫蛋白质的成功应用<35>,使我们有可能对这种寄生原虫进行示踪实验。该方法也可用于抗弓形虫药物的筛选。

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Endo,T.and Yagita,K: "Toxoplasma gondii : A simple method for titration of infectivity with monolayer cells." Japan.J.Med.Sci.Biol.42. 13-23 (1989)
Endo,T. 和 Yagita,K:“弓形虫:单层细胞感染性滴定的简单方法。”
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    0
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Endo T.& Yagita K.: "Changes in internal pH and in vitro amino and uptake by Toxoplasma gondii" J.Protozool.
远藤 T.
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Endo,T.and Yagita,K.: "Effect of Ext ellulan ions on motility and cell entry in Toxoplasma gondii" J.Protozoology. 37. 133-138 (1990)
Endo,T. 和 Yagita,K.:“Ext ellulan 离子对弓形虫运动和细胞进入的影响”J.Protozoology。
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    0
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Yasuda, T., Yagita, K., Nakamura, T. and Endo, T.: "Immunocytochemical localization of actin in Toxoplasma gondii." Parasitol. Res.75. 107-113 (1988)
Yasuda, T.、Yagita, K.、Nakamura, T. 和 Endo, T.:“弓形虫中肌动蛋白的免疫细胞化学定位。”
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ENDO Takuro其他文献

ENDO Takuro的其他文献

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{{ truncateString('ENDO Takuro', 18)}}的其他基金

A Study for Developing Educational Materials of the Oriental Bodywork Methods: Searching their Possibility as Educational Materials for Physical Education
东方车身方法教材开发研究:探索其作为体育教材的可能性
  • 批准号:
    21500551
  • 财政年份:
    2009
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research and Development of Bibliographic Database on Sport Philosophy
体育哲学书目数据库的研究与开发
  • 批准号:
    61580092
  • 财政年份:
    1986
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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