Genes in the HLA class III region and their products in patients with rheumatoid arthritis and Sjogren's syndrome.

类风湿关节炎和干燥综合征患者 HLA III 类区域的基因及其产物。

• 批准号: 63570301
• 负责人: MORIUCHI Junko
• 金额: $ 1.34万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570301/
• 关键词: HLA; Class III region; restriction fragment length polymorphism; rheumatoid arthritis; Sjogren's syndrome; complement component 4; 21-hydroxylase; Southern hybridization; 慢性関節リウマチ; HLA、クラスIII; 捕体蛋白; シェーグレン症候群; クラスIIIサザンブロット; C4A; C4B

Molecular biological analysis of DNA and the allotypes of the fourth component of complement (C4) and Factor B (Bf) were studied in patients with Sjogren's syndrome (SS) and rheumatoid arthritis (RA).The result we obtained were: 1. There is a well documented awqssociation between RA and HLA- DR4 and DRw53 in many racial groups. In this study, we observed a significantly increased frequency of C4B5 in RA patients, especially in male patients. Since a strong linkage disequilibrium among genes of C4B5, DR4 and DRw53 is reported in Japanese, and since RA patients in other ethnics are not associated with C4B5, the C4BS was supposed not to have a direct role for the development of RA. 2. C4A null (C4AQO) was frequently observed in SS patients without other autoimmune disease (primary SS). By Southern blot analysis using CDNA probes for the 5' end of C4 and CyP21 and four restriction enzymes, genomic DNA from patients and controls with or without C4AQO were studied. The results suggest that most examples of C4AQO in the Japanese are not due to a major deletion of the C4A gene.

本文对干燥综合征（SS）和类风湿关节炎（RA）患者的DNA及补体第四成分（C_4）和B因子（Bf）进行了分子生物学分析。在许多种族群体中，RA与HLA-DR 4和DRw 53之间存在着广泛的相关性。在这项研究中，我们观察到C4 B5在RA患者中的频率显著增加，尤其是在男性患者中。由于日本人报道C4 B5、DR 4和DRw 53基因之间存在强烈的连锁不平衡，而其他种族的RA患者与C4 B5无关，因此C4 BS被认为对RA的发生没有直接作用。2. C4 A null（C4 AGO）常见于无其他自身免疫性疾病的SS患者（原发性SS）。用C4和CyP 21的5'端cDNA探针和4种限制性内切酶对C4 AGO阳性和阴性患者的基因组DNA进行Southern印迹分析。结果表明，日本人中的大多数C4 AKO病例不是由于C4 A基因的主要缺失。

项目成果

J. Moriuchi, K. Morita, Y. Ichikawa, M. Takaya, H. Shimizu, M. Uchiyama, S. Arimori, K. Sato, K. Tsuji, M. Maeda, N. Uryu: "Genes in the HLA class III region and their products in patients with rheumatoid arthritis." Jpn. J. Clin. Immun. 12-4 394-440, 198

J. Moriuchi、K. Morita、Y. Ichikawa、M. Takaya、H. Shimizu、M. Uchiyama、S. Arimori、K. Sato、K. Tsuji、M. Maeda、N. Uryu：“HLA 类基因

J.Moriuchi,et al.: The 6th SEAPAL Congress of Rheumatology.121 (1988)

J.Moriuchi 等人：第 6 届 SEAPAL 风湿病学大会.121 (1988)

J. Moriuchi, Y. Ichikawa, M. Takaya, H. Shimizu, H. Inoko, K. Tsuji, A. Wakisaka, R. Dawkins, S. Arimori: "Association of complement allele, C4AQ0, in Japanese patients with primary Sjogren's syndrome." Arthritis Rheum.

J. Moriuchi、Y. Ichikawa、M. Takaya、H. Shimizu、H. Inoko、K. Tsuji、A. Wakisaka、R. Dawkins、S. Arimori：“日本原发性干燥病患者中补体等位基因 C4AQ0 的关联

J.Moriuchi,et al.: Arthritis and Rheumatism.

J.Moriuchi 等人：关节炎和风湿病。

森田和之 等: リウマチ.

森田和之等：风湿病。