Role of Retinal Pigment Epithelium in Development and Healing of Retinal Detachment

视网膜色素上皮（RPE）对视网膜的正常定位和视网膜脱离的愈合过程具有重要作用。本实验研究活体眼视网膜粘合力、全视网膜光凝对视网膜电图c波的影响以及眼缺血对RPi功能和形态的影响。用新研制的微压测量系统记录分离视网膜的跨壁压力，测量感觉视网膜与RPE之间的粘附力。估计兔活眼视网膜粘合力为（1.8<正负>）。2) x10 ^2dyn/cm，通过光凝、冷冻固定、热疗、静脉注射乙酰唑胺等方法提高粘合力。在全视网膜光凝前后记录染色兔眼dc登记的电脑波。按凝血严重程度分为三组。凝血后3 d各组ERG c波均消失，c波恢复的方式表明c波振幅的最终恢复程度和RPE屏障功能重建所需的持续时间都与凝血面积有关。将眼内压升高到收缩压以上，可诱导兔眼急性视网膜缺血。分别在缺血前、缺血中、缺血后记录ERG，光镜、电镜观察视网膜和RPE的结构变化。发现眼缺血可诱导RPE增殖。

The retinal pigment epithelium (RPE) has a important role for normal aposition of retina, and for healing process of retinal detachment. In this study, the retinal adhesive force in living eye, the effect of panretinal photocoagulation on electroretinographic c wave and the effect of ocular ischemia on RPi function and morphology.The adhesive force between sensory retina and RPE was measured by recording transmural pressure across detached retina with a newly developed micropressure measuring system. The retinal adhesive force of rabbit living eye was estimated to be (1.8<plus-minus>O. 2)x1O^2dyn/cm, and the adhesive force was increased by photocoagulation, cryoretinopexy, diathermy and intravenous acetazolamide injection.DC-registerd ERG c waves were recorded from pigmented rabbit eyes before and after panretinal photocoagulation. The rabbits were subdivided into three groups according to severity of coagulation. The ERG c waves were found to disappear 3 days after coagulation in all groups, and recovery of c wave was observed in a manner suggesting that the ultimate extent of recovery in the c wave amplitude and the duration required for the re-establishment of RPE barrier function both depend on coagulated area.Acute retinal ischemia was induced in rabbit eyes by raising the intraocular pressure above systoric blood pressure. ERG was recorded before, during and after ischemia and structural changes of retina and RPE were observed using light and electron microscopies. Ocular ischemia was found to induce RPE proliferation.