Mechanism of Bacterial Cell Flocculation by Extracellular Materials from Nocardia Amarae.

阿马拉诺卡氏菌胞外物质对细菌细胞絮凝的机制。

• 批准号: 01550764
• 负责人: KOIZUMI Junichi
• 金额: $ 0.26万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01550764/
• 关键词: Bacterial cell flocculation; Peptidic bioflocculant; Nocardia amarae; Extracellular materials; Synergentic flocculation; ノカルディアアマラエ; ノカルディア; 凝集; フロック形成; 協同作業; 環境微生物学

The fact that Nocardia amarae YK1 produced bacterial flocculation-inducing substances (FIX : Flocculation Inducer-X) was discovered. FIX had a function of flocculating the proliferating cells ; FIX-induced flocculation was retarded in resting cell conditions.FIX had a broad spectrum on flocculation induction from gram-negative to -positive bacteria. In the presence of FIX, Achromobacter cycloclastus IAM1013, Acinetobacter calcoaceticus IAM1517, Bacillus subtilis IAM1069, Escherichia coliC600-1, E. coli IAM1239, Flavobacterium lutescens IAM1667, Klebsiella pneumoniae IAM1102, Micrococcus luteus IAM1313 and Pseudomonas putida IAM1002 formed flocs, respectively, though B. cereus IAM1029 exhibited no flocculation.The fractionation of bioflocculant FIX produced by Nocardia amarae YK1 revealed that the flocculant was a mixture of more than three substances. Although individual fractions did not exhibit the ability of flocculant at pH 5 to 7, synergetic flocculation was observed at pH 7 after mixing the fractions. Infrared spectrophotometry suggested that the chief components of the frctions might be peptides. Enzymatic digestion of peptide moiety results in disappearance of flocculation activity.

发现了诺卡氏菌YK1产生细菌絮凝诱导物质(FIX：FIZATION INDIRECTER-X)的事实。FIX具有絮凝增殖细胞的功能；FIX诱导的絮凝在静息细胞条件下被延缓；FIX对革兰氏阴性菌到阳性菌的絮凝诱导作用范围广泛。在FIX的存在下，环状不动杆菌IAM1013、醋酸钙不动杆菌IAM1517、枯草芽孢杆菌IAM1069、大肠杆菌IAM1239、黄杆菌IAM1667、肺炎克雷伯菌IAM1102、黄色微球菌IAM1313和恶臭假单胞菌IAM1002分别形成絮体，蜡状芽胞杆菌IAM1029无絮凝。虽然各组分在pH值为5~7时不具有絮凝剂的作用，但在pH值为7时，各组分混合后表现出协同絮凝作用。红外光谱分析表明，其主要成分可能是多肽。多肽部分经酶消化后，絮凝活性消失。

项目成果

小泉 淳一・武田 穣: "Nocardia amaraeの産生する複数の凝集関与物質とそれらの協同作業によるフロック形成" 水質汚濁研究. 13. 288-292 (1990)

Junichi Koizumi 和 Jo Takeda：“诺卡氏菌产生的多种絮凝相关物质以及他们合作形成的絮凝体”《水污染研究》13. 288-292 (1990)。

Kiyoshi TODA et al.: "Functions of Extracellular polymeric materials and their Applications." Hakko Kogaku Kaishi. 68. 301-318 (1990)

Kiyoshi TODA 等人：“细胞外聚合物材料的功能及其应用”。

小泉 淳一: "細胞外バイオポリマ-の機能と利用ーバイオフロキュラントとバイオサ-ファクタント" 微生物. 5. 545-551 (1989)

Junichi Koizumi：“细胞外生物聚合物的功能和用途 - 生物絮凝剂和生物表面活性剂”微生物。5. 545-551 (1989)

小泉 淳一: "Nocardia amarae の産生する複数の凝集関与物質とそれらの協同作業によるフロック形成" 水質汚濁研究. 13. 288-292 (1990)

Junichi Koizumi：“由诺卡氏菌产生的多种絮凝相关物质及其协同作用形成絮凝体”《水污染研究》，13. 288-292 (1990)。

Jun-ichi KOIZUMI and Minoru TAKEDA: "Synergetic Flocculation by Bioflocculants FIX that Were Extracellularly Produced from Nocardia amarae." Suisitu Odaku Kenkyuu. 13. 288-292 (1990)

Jun-ichi KOIZUMI 和 Minoru TAKEDA：“由阿马拉诺卡氏菌细胞外产生的生物絮凝剂 FIX 进行协同絮凝。”