Bioorganically Chemical Studies on Abscisic Acid Analogs Produced by a Fungus.

1)(&lt；正负&gt；)-4^‘-羟基-γ-亚氨基乙酸；以异佛尔酮为原料合成了3，3，5-三甲基-4-环己烯基乙酸酯；2)手性合成酮；(S)-2，2-二甲基-4-羟基-1-环己酮。(正负)-2，2-二甲基-1，4-环己二酮的缩醛。将2，2-二甲基环己酮与大肠杆菌pJp43进行微生物羟基化反应，得到(S)-酮醇。以(R)-2，2-二甲基-4-羟基-1-环己酮为原料，合成了天然的4^‘-羟基和1^’，4‘-二羟基-γ-硫代亚氨基乙酸。4)研究了(R)-2，2-二甲基-4-羟基-1-环己酮的植物生理活性。此外，还筛选了壳霉ABA生物合成的抑制剂。对ABA的3‘-氧代类似物进行了合成和生物活性测定。

1) (<plus-minus>)-4^' -hydroxy-gamma-ionylideneacetic acid ; a analog of abscisic acid (ABA) produced a fungus Cercospora cruenata, was synthesized from 3, 3, 5-trimethy1-4-cyclohexenyl acetate, which was prepared from isophorone.2) A chiral synthone ; (S) -2, 2-dimethy1-4-hydroxy-1-cyclohexanone was prepared by yeast reduction of 1-ethyleneglycol. Acetal of (<plus-minus>) -2, 2-di menthy1-1, 4-cyclohexadione. Also, Microbial hydroxylation of 2, 2-dimethylcyclohexanone with Escherichia coli pJp43 gave the (S) -Ketol. sn2 reaction of the (S) -ketol by Mitsunobu method afforded the (R) -keto3) Biosynthetic intermediates of ABA in C. cruenta ; natural 4^' -hydroxy- and 1^',4^' -dihydroxy- gamma-ionylideneacetic acid were synthesized from (R) -2, 2-dimethy1-4-hydroxy-1-cyclohexanone.4) Plant physiological activities of ABA analogs produced by C. cruenta were examined. Also, Inhibitors of ABA biosynthesis in C. cruenta were selected. Synthesis and bioassay of 3^'-oxo analog of ABA were performed.