Analytical Study of Restriction of Measles Virus M Protein Synthesis at Elevated Temperatures

高温下限制麻疹病毒 M 蛋白合成的分析研究

• 批准号: 01570253
• 负责人: OGURA Hisashi
• 金额: $ 1.34万
• 依托单位: Kanazawa University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570253/
• 关键词: Measles Virus; M Protein; Elevated Temperatures; Gene Transfer; Transient Expression; 麻疹ウィルス

We analyzed a phenomenon that selective translational inhibition of measles virus Membrane (M) protein synthesis in the infected cells at elevated temperatures by transfection of measles virus fullーlength cDNA clones. We used four kinds of eukaryotic expression vectors for transient expression of measles virus Hemagglutinin (H) and M proteins. Among them, only pSG5 vector under the control of the SV40 early promoter was expressible. H protein was well expressed in the H cDNA-transfected COS-1 cells at both 35^ﾟC and 39^ﾟC. On the other hand, although M protein was expressed in the M cDNA-transfected cells at 35^ﾟC, its synthesis became undetected immediately after shiftーup to 39^ﾟC in spite of the presence of the M mRNA. Thus, it was demonstrated that the selective translational inhibition of M protein synthesis is reprosuced in the M cDNA-transfected cells by temperature elevation in the absence of the other MV specific RNAs and proteins.

我们通过转染麻疹病毒全长cDNA克隆，分析了在高温下选择性翻译抑制麻疹病毒膜蛋白（M蛋白）合成的现象。本研究采用四种真核表达载体瞬时表达麻疹病毒血凝素（H）和M蛋白。其中，只有在SV 40早期启动子控制下的pSG 5载体是可表达的。在35 ℃和39 ℃下，H蛋白在转染H cDNA的COS-1细胞中均得到了良好的表达。另一方面，尽管M蛋白在35 ℃时在M cDNA转染的细胞中表达，但在温度升至39 ℃后，尽管存在M mRNA，但其合成立即变得不可检测。因此，证明了在不存在其它MV特异性RNA和蛋白质的情况下，通过温度升高在M cDNA转染的细胞中再现了M蛋白合成的选择性翻译抑制。

项目成果

H.Ogura: "Inhibition of peptide chain eleongation of measles virus membrane protein by temperature elevation" Joural of Virology.

H.Ogura：“温度升高对麻疹病毒膜蛋白肽链延长的抑制”《病毒学杂志》。

H. Ogura: "Replication of measles virus in Vero cells at elevated temperature" Microbiology and Immunology. 33(10). 907-914 (1989)

H. Ogura：“高温下麻疹病毒在 Vero 细胞中的复制”微生物学和免疫学。

H. Ogura: "Longーterm effect of elevated temperature of SSPE virus expression in persistently infected rat glial cells" Archives of Virology. 104(3-4). 259-269 (1989)

H. Ogura：“持续感染的大鼠神经胶质细胞中 SSPE 病毒表达的高温的长期影响”病毒学档案 104(3-4) (1989)。

H.Ogura: "Glycosylation processing of measles virus haemagglutinin protein in infected cells" Journal of General Virology.

H.Ogura：“受感染细胞中麻疹病毒血凝素蛋白的糖基化处理”普通病毒学杂志。

H.Ogura: "Long-term effect of elevated temperatures on SSPE virus expression in persistently infected rat glial cells" Archives of Virology. 104. 259-269 (1989)

H.Ogura：“高温对持续感染的大鼠神经胶质细胞中 SSPE 病毒表达的长期影响”病毒学档案。