Immunogenicity, Pathogenicity, and Epidemiological Application of Rotavirus Protective Antigens
轮状病毒保护性抗原的免疫原性、致病性和流行病学应用
基本信息
- 批准号:01570256
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Nucleotide Sequence determination of the 4th RNA segment of SA11 clones with different plaque size identified five amino acids difference between large (L2) and small (S1) clones. In the mice administered orally with L2 clone, incubation time for diarrhea was shorter and the degree of diarrhea was severer than in those infected with S1 clone.2. In a competitive immunoassay using serotype-specific and cross-reactive neutralizing monoclonal antibodies directed at VP4 and VP7, antibody response to VP7 epitopes of the infecting serotype of the virus was found in both infants and children in a high frequency. In contrast, antibody response to VP4 and to heterotypic VP7 was observed only when the individuals possessed antibodies to any serotype of rotavirus in their acute-phase or pre-vaccination sera.3. We sequenced the genes coding for VP4 and VP7 of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L … More 26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established serotypes. This finding, together with the previous serological characterizations, suggests that the VP7 (G) serotype of the L26 and L27 strains is distinct from those of strains of the previously established serotypes. In contrast, the VP4 sequences of the L26 and L27 strains were quite similar to those of virulent serotype 2 strains. RNA-RNA hybridization tests indicated an overall genetical relationship of the L26 and L27 strains with serotype 2 human strains.4. Antigenic and genetic properties of non-serotype 6 bovine rotaviruses isolated in Thailand were studied by cross-neutralization tests, nucleotide sequence determination of VP7 gene, and RNA-RNA hybridization. Two strains (61A and A44) were serologically and genetically related to strain KK3 of serotype 10. In contrast, A5 strain was found to be antigenically similar to human serotype 8 strain 69M. VP7 sequence analysis confirmed this finding. These bovine strains exhibited a moderate overall genetic relatedness with serotype 2 and serotype 8 human rotavirus strains. Less
1.对不同噬菌斑大小的SA 11克隆的第4个RNA片段进行核苷酸序列测定,发现大(L2)和小(S1)克隆之间存在5个氨基酸差异。经口感染L2克隆的小鼠腹泻潜伏期较短,腹泻程度较重.在使用针对VP 4和VP 7的病毒型特异性和交叉反应性中和单克隆抗体的竞争性免疫测定中,在婴儿和儿童中发现了对感染病毒血清型的VP 7表位的抗体应答。相反,只有当个体在其急性期或接种前血清中具有针对任何血清型的轮状病毒的抗体时,才观察到对VP 4和异型VP 7的抗体应答。我们对人轮状病毒L26和L27株的VP 4和VP 7基因进行了序列测定,这两株病毒具有I亚群特异性,但RNA模式较长。推导的L株的VP 7氨基酸序列 ...更多信息 26和L27与已建立的轮状病毒血清型的同源性较低(73.6 ~ 81.9%)。这一发现以及先前的血清学表征表明,L26和L27菌株的VP 7(G)血清型与先前确定的血清型菌株的血清型不同。与此相反,L26和L27株的VP 4序列与强毒株血清2型的VP 4序列非常相似。RNA-RNA杂交试验表明L26和L27菌株与血清2型人类菌株的总体遗传关系。通过交叉中和试验、VP 7基因核苷酸序列测定和RNA-RNA杂交,对泰国分离的非血清型6型牛轮状病毒的抗原性和遗传特性进行了研究。61 A和A44与血清型10的KK 3株有血清学和遗传学上的亲缘关系。相反,发现A5菌株与人血清型8菌株69 M抗原性相似。VP 7序列分析证实了这一发现。这些牛病毒株与血清2型和血清8型人轮状病毒株表现出中度总体遗传相关性。少
项目成果
期刊论文数量(29)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Taniguchi, K., Pongsuwanna, Y., Choonthanom, M., and Urasawa, S.: "Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6." Nucleic Acids Res.18. 4614 (1990)
Taniguchi, K.、Pongsuwanna, Y.、Choonthanom, M. 和 Urasawa, S.:“具有与血清型 6 不同的血清型特异性的牛轮状病毒(61A 株)的 VP7 基因的核苷酸序列。”
- DOI:
- 发表时间:
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- 影响因子:0
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Yaowapa Pongsuwanne et al.: "Subgroup and serotype distributions of human,bovine,and porcine rotavirus in Thailand" Journal of Clinical Microbiology. 27. 1956-1960 (1989)
Yaowapa Pongsuwanne 等人:“泰国人、牛和猪轮状病毒的亚组和血清型分布”临床微生物学杂志。
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Urasawa, S. Urasawa, T. Wakasugi, F., Kobayashi, N., Taniguchi, K., Lintag, I. C., Saniel, M. C., and Goto, H.: "Presumptive seventh serotype of human rotavirus." Arch. Virol. 113. 279-282 (1990)
Urasawa, S. Urasawa、T. Wakasugi, F.、Kobayashi, N.、Taniguchi, K.、Lintag, I. C.、Saniel, M. C. 和 Goto, H.:“人类轮状病毒的假定第七血清型”。
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- 影响因子:0
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Taniguchi, K., Nishikawa, K., Kobayashi, N., Urasawa, T., Gorziglia, M., Urasawa, S.: "Association of plaque size with VP4 sequence of SA11 virus clones." Arch. Virol.
Taniguchi, K.、Nishikawa, K.、Kobayashi, N.、Urasawa, T.、Gorziglia, M.、Urasawa, S.:“噬斑大小与 SA11 病毒克隆的 VP4 序列的关联。”
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- 影响因子:0
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Koki Taniguchi et al.: "Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6" Nucleic Acids Research. 18. 4613- (1990)
Koki Taniguchi 等人:“具有与血清型 6 不同的血清型特异性的牛轮状病毒(61A 株)的 VP7 基因的核苷酸序列”核酸研究。
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TANIGUCHI Koki其他文献
TANIGUCHI Koki的其他文献
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{{ truncateString('TANIGUCHI Koki', 18)}}的其他基金
Development of the system for the formation of infectious particles of rotavirus with the aid of genetic engineering
借助基因工程开发轮状病毒感染性颗粒形成系统
- 批准号:
14370105 - 财政年份:2002
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on the structure and function of viral proteins using artificial particles of human rotavirus
利用人轮状病毒人工颗粒研究病毒蛋白的结构和功能
- 批准号:
10470080 - 财政年份:1998
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
STUDIES ON THE STRUCTURE AND FUNCTION OF NONSTRUCTURAL PROTEINS OF ROTAVIRUS
轮状病毒非结构蛋白的结构和功能研究
- 批准号:
07457080 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of poliovirus-rotavirus chimeric vaccine by using poliovirus artificial interfering particle RNA
利用脊髓灰质炎病毒人工干扰颗粒RNA研制脊髓灰质炎病毒-轮状病毒嵌合疫苗
- 批准号:
04557025 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)