Immunogenicity, Pathogenicity, and Epidemiological Application of Rotavirus Protective Antigens

1.对不同斑块大小的SA11克隆的第4个RNA片段进行核苷酸序列测定，发现大克隆(L2)和小克隆(S1)的5个氨基酸序列存在差异。结论：1.口服L2克隆组小鼠腹泻潜伏期较S1克隆组短，腹泻程度重。在针对VP4和VP7的血清型特异性和交叉反应中和单抗的竞争性免疫分析中，在婴儿和儿童中发现对该病毒感染血清型的VP7表位的抗体反应频率很高。相反，只有当个体在急性期或接种前的血清中对任何血清型轮状病毒具有抗体时，才能观察到对VP4和异型VP7的抗体应答。我们对人轮状病毒L26和L27株VP4和VP7的编码基因进行了序列测定，这些基因具有亚群I特异性，但具有长RNA模式。L…株VP7氨基酸序列的推导26株和L27株与已建立的血清型轮状病毒株同源性较低(73.6%~81.9%)。这一发现与先前的血清学特征一起表明，L26和L27毒株的VP7(G)血清型与以前建立的血清型毒株的VP7(G)型不同。通过交叉中和试验、VP7基因核苷酸序列测定和RNA-RNA杂交，对泰国分离的非6型牛轮状病毒的抗原性和遗传特性进行了研究。两株病毒(61a和A44)在血清学和基因上与10型KK3株有亲缘关系，而A5株与人8型69M株在抗原性上相似。VP7序列分析证实了这一发现。较少

1. Nucleotide Sequence determination of the 4th RNA segment of SA11 clones with different plaque size identified five amino acids difference between large (L2) and small (S1) clones. In the mice administered orally with L2 clone, incubation time for diarrhea was shorter and the degree of diarrhea was severer than in those infected with S1 clone.2. In a competitive immunoassay using serotype-specific and cross-reactive neutralizing monoclonal antibodies directed at VP4 and VP7, antibody response to VP7 epitopes of the infecting serotype of the virus was found in both infants and children in a high frequency. In contrast, antibody response to VP4 and to heterotypic VP7 was observed only when the individuals possessed antibodies to any serotype of rotavirus in their acute-phase or pre-vaccination sera.3. We sequenced the genes coding for VP4 and VP7 of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L … More 26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established serotypes. This finding, together with the previous serological characterizations, suggests that the VP7 (G) serotype of the L26 and L27 strains is distinct from those of strains of the previously established serotypes. In contrast, the VP4 sequences of the L26 and L27 strains were quite similar to those of virulent serotype 2 strains. RNA-RNA hybridization tests indicated an overall genetical relationship of the L26 and L27 strains with serotype 2 human strains.4. Antigenic and genetic properties of non-serotype 6 bovine rotaviruses isolated in Thailand were studied by cross-neutralization tests, nucleotide sequence determination of VP7 gene, and RNA-RNA hybridization. Two strains (61A and A44) were serologically and genetically related to strain KK3 of serotype 10. In contrast, A5 strain was found to be antigenically similar to human serotype 8 strain 69M. VP7 sequence analysis confirmed this finding. These bovine strains exhibited a moderate overall genetic relatedness with serotype 2 and serotype 8 human rotavirus strains. Less