Experimental Research for Mechanism of Brain Abnormalities Induced by Intrauterine Infection with Cytomegalovirus.

巨细胞病毒宫内感染致脑异常机制的实验研究。

• 批准号: 01570264
• 负责人: TSUTSUI Yoshihiro
• 金额: $ 0.9万
• 依托单位: Institute for Developmental Research, Aichi Prefectural Colony.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570264/
• 关键词: cytomegalovirus; intrauterine infection; brain abnormalities; サイトメガロウィルス

Brains of mouse fetus were infected with murine cytomegalovirus (MCMV) by intraventricular injection with MCMV on day 15 of gestation. At first. We have done neuropathological analysis of the brains of the offspring after birth. We found that some of the neurons were susceptible to MCMV infection, especially in the cerebral cortex and the hippocampus by immunohistochemical double staining using antibodies to neuron-specific enolase and antibodies to the immdiate-early antigen of MCMV. Cystic brain lesions in the cortex and the white matter were observed in about 20% of the MCMV-infected mouse 3 to 4 weeks after birth. As perivascular caffings were observed around the cystic lesions, we performed the immunohistochemical double staining using antibodies to human factor VIII and the anti-MCMV antibodies. We found that some of the endothelial cells of the brain blood vessels were infected with MCMV. Therefore, it is possible that cystic lesions may be formed by ischemic consequences after infection of the vessels with MCMV.

用小鼠巨细胞病毒（MCMV）脑室注射法感染孕15天胎鼠脑。一开始我们对出生后的后代的大脑进行了神经病理学分析。我们发现，一些神经元是易感MCMV感染，特别是在大脑皮层和海马免疫组化双重染色使用的抗体，神经元特异性烯醇化酶和抗体的MCMV的立即早期抗原。出生后3 - 4周，在约20%的MCMV感染小鼠中观察到皮质和白色物质中的囊性脑病变。由于囊状病变周围可见血管周围包绕，我们用抗人因子VIII抗体和抗MCMV抗体进行免疫组化双重染色。我们发现脑血管的一些内皮细胞被MCMV感染。因此，MCMV感染血管后的缺血性后果可能会形成囊性病变。

项目成果

山本 直彦,山田 善也,大黒 徹,西山 幸廣,筒井 祥博他: "Antiviral effect of oxetanocin G against guinea pig cytomegalovirui infection in vifro and in vivo." Journal of Antibiotics. 18. 1573-1578 (1990)

Naohiko Yamamoto、Yoshiya Yamada、Toru Daikoku、Yukihiro Nishiyama、Yoshihiro Tsutsui 等人：“Oxetanocin G 对体外和体内豚鼠巨细胞病毒感染的抗病毒作用”，《抗生素杂志》18。1573-1578。

筒井 祥博,山崎 義朗: "Subcellular distribntion of the major immediate early protein of human cytomegalovirus changes during infection." Journal of General Virology.

Yoshihiro Tsutsui、Yoshiro Yamazaki：“感染期间人类巨细胞病毒主要立即早期蛋白的亚细胞分布发生变化。”

Tsutsui, Y., Kashiwai, A. et al.: "Susceptibility of brain cells to murine cytomegalovirus infection in the developing mouse brain." Acta Neuropathl. (Berl). 79. 262-270 (1989)

Tsutsui, Y., Kashiwai, A. 等人：“发育中的小鼠大脑中脑细胞对鼠巨细胞病毒感染的敏感性。”

筒井祥博: "サイトメガロウィルスによる発育期マウス脳障害の細胞学的解析" 厚生省精神・神経疾患研究委託事業報告書 鴨下印班 「発育期脳障害の発生予防と成因に関する研究」. 149-156 (1990)

Yoshihiro Tsutsui：“巨细胞病毒引起的发育性小鼠脑损伤的细胞学分析”厚生劳动省精神和神经疾病研究委托项目 Kamoshita Seal Group“发育性脑损伤的预防和病因学研究”149-156。 (1990)

山本 直彦,山田 善也,大黒 徹,西山 幸廣,筒井 祥博,島田 信義,高橋 克健: "Anfiviral effect of oxetanocin G againt guiner pigcytomegnlovirus infection in vitro and in vivo" Journal of Antibitics. 18. 1573-1578 (1990)

Naohiko Yamamoto、Yoshiya Yamada、Toru Daikoku、Yukihiro Nishiyama、Yoshihiro Tsutsui、Nobuyoshi Shimada、Katsuhiro Takahashi：“Oxetanocin G 对吉纳猪巨细胞病毒感染的体外和体内抗病毒作用”《抗生素杂志》18。1573-1578（1990）。