Regulation of Type I Collagen Gene Expression in Fibrotic Skin Disorders

纤维化皮肤病中 I 型胶原蛋白基因表达的调节

• 批准号: 01570580
• 负责人: HATAMOCHI Atsushi
• 金额: $ 1.34万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570580/
• 关键词: Type I collagen; Gene expression; Transcription; Scleroderma; Werner's syndrome; Transformed; fibroblasts; Cutis laxa; 転写因子; プロモ-タ-

To a large extent the macromolecular organization and physiological function of connective tissue depends on the selection of the right collagen types. For the normal functioning of connective tissue an exact control of collagen gene expression is therefore required. Studies using cultured skin fibroblasts have already shown that fibroblasts from the involved skin from scleroderma and keloid patients show increased collagen synthesis as compared with those from normal controls. We studied collagen gene expression in dermal fibroblasts from patients with morphea and Werner's syndrome and transformed human fibroblasts. The level of m-RNA for type I collagen in fibroblasts derived from the inflammatory lesion were approximately 60% above those obtained from the normal looking lesion of the skin of a patient with morphea. Increased collagen synthesis accompanying elevated m-RNA levels incultured Werner's syndrome was observed. Decreased type I and type III collagen RNA transcription was observed in transformed human fibroblasts. We also studied collagen metabolism in cutis laxa fibroblasts. And we observed that increased collagenase gene expression associated with unaltered expression of type I and type III collagen.

结缔组织的大分子组织和生理功能在很大程度上取决于胶原蛋白类型的选择。因此，为了结缔组织的正常功能，需要对胶原基因的表达进行精确的控制。使用培养的皮肤成纤维细胞的研究已经表明，硬皮病和瘢痕疙瘩患者受累皮肤的成纤维细胞与正常对照相比，胶原合成增加。我们研究了吗啡和Werner综合征患者真皮成纤维细胞和转化的人成纤维细胞中胶原基因的表达。来自炎性病变的成纤维细胞中I型胶原的m-RNA水平大约比来自吗啡患者正常皮肤病变的m-RNA水平高出60%。在培养的Werner综合征中观察到胶原合成增加并伴随m-RNA水平升高。在转化的人成纤维细胞中，观察到I型和III型胶原RNA转录降低。我们还研究了皮肤松弛成纤维细胞的胶原代谢。我们观察到胶原酶基因表达的增加与I型和III型胶原的表达没有变化相关。

项目成果

Atsushi Hatamochi,Masashi Ono,et al.: "Regulation of collagen gene expression by transformed human fibroblasts:Decreased type I and type III collagen RNA transcription" The Journal of Investigative Dermatology. 96. (1991)

Atsushi Hatamochi、Masashi Ono 等人：“转化人成纤维细胞对胶原蛋白基因表达的调节：I 型和 III 型胶原蛋白 RNA 转录减少”《皮肤病学研究杂志》。

Masami Arakawa, Atsushi Hatamochi, et al.: "Increased collagen synthesis accompanying elevated m-RAN levels in cultured Werner's syndrome fibroblasts" The Journal of Investigative Dermatology. 94. 187-190 (1990)

Masami Arakawa、Atsushi Hatamochi 等人：“培养的维尔纳综合征成纤维细胞中胶原蛋白合成增加，同时 m-RAN 水平升高”《皮肤病学研究杂志》。

Atsushi Hatamochi,Tamiko Wada,et al.: "Collagen metabolism in cutis laxa fibroblasts:Increased collagenase gene expression associated with unaltered expression of type I and type III collagen" The Journal of Investigative Dermatology.

Atsushi Hatamochi、Tamiko Wada 等人：“皮肤松弛成纤维细胞中的胶原代谢：与 I 型和 III 型胶原表达不变相关的胶原酶基因表达增加”《皮肤病学研究杂志》。

Atushi Hatamochi,Tamiko Wada,et al.: "Collagen metabolism in cutis laxa fibroblasts:Increased collagenase gene expression associated with unaltered expression of type I and type III collagen" The Journal of Investigative Dermatology.

Atushi Hatamochi、Tamiko Wada 等人：“皮肤松弛成纤维细胞中的胶原代谢：与 I 型和 III 型胶原表达不变相关的胶原酶基因表达增加”《皮肤病学研究杂志》。

Atsushi Hatamochi, Masashi Ono, et al.: "Analysis of collagen gene expression by cultured morphea fibroblasts" British Journal of Dermatology.

Atsushi Hatamochi、Masashi Ono 等人：“培养的硬斑病成纤维细胞的胶原蛋白基因表达分析”英国皮肤病学杂志。