Influence of Fibroblasts on Chemosensitivity for Lung Cancer and Clinical Applications

为了研究成纤维细胞对肺癌细胞的影响，进行了体外MTT比色法测定其对抗癌药物的敏感性，以及裸鼠移植瘤的体内研究，并探讨了可能的临床应用。发现敏感性是异质性的。将肺癌细胞和成纤维细胞混合在培养物中（1：1），孵育3天，并检查对CDDP的敏感性。在18种培养物中的11种中观察到相互作用，但当在混合培养物的同时加入CDDP时，在这些培养物中的任何一种中均未观察到相互作用。因此，成纤维细胞条件培养基中的另一个因子似乎影响培养中的肺癌细胞，其作用被热处理破坏，但不受辐射影响。在体内研究中，当成纤维细胞加入到移植到裸鼠体内的肺癌细胞中时，肿瘤更容易发生，并且它们的生长也更快。在临床上，涉及促进肺癌细胞增殖的成纤维细胞的病例通常在相对早期阶段显示出向淋巴结的广泛转移。因此，某些类型的成纤维细胞似乎是影响肺癌细胞增殖以及影响抗癌剂敏感性的因素。作为体外试验，成纤维细胞的条件培养基对肺癌细胞（Luci 10）的影响可适用于预测个体肺癌患者的预后。

To examine the influence of fibroblasts on lung cancer cells, in vitro MTT colorimetric assay was conducted on their sensitivity toward anticancer agents as well as an in vivo study on the development of tumors transplanted in nude mice and possible clinical applications were investigated. Sensitivity was found to be heterogenous. Lung cancer cells and fibroblasts were combined in a culture ( 1 : 1 ), incubated for 3 days, and examined for sensitivity to CDDP. A mutual effect was observed in 11 out of 18 cultures, but not in any of these cultures when CDDP was added at the same time the culture was mixed. Another factor in the conditioned medium by fibroblasts thus appered to influence lung cancer cells in culture and whose action is destroyed by heat processing, but not affected by radiation. In the in vivo study, tumor development occurred more easily when fibroblasts were added to the lung cancer cells transplanted in to the nude mouse, and their growth was also greater. Clinically speaking, cases involving fibroblasts which promote proliferation of the lung cancer cells often show widespread metastasis to the lymph nodes at a comparatively early stage. Some types of fibroblasts thus appear to be factors influencing the proliferation of lung cancer cells as well as affecting sensitivity to anticancer agents. As an in vitro test, the influence of the conditioned medium by fibroblasts on lung cancer cells (Luci 10) may be applicable for predicting the prognosis of individual patients with lung cancer.