Receptor Analysis of the Cholinergic and Dopaninergic Neuronal Graft in the Central Nervous System-in Vitro Macro-Autoradiographical Investigation

A.多巴胺能移植例：将儿茶酚胺产生细胞(胎儿中脑植入细胞悬液)移植到左侧黑质注射6-OHDA诱导的偏侧帕金森病大鼠的同侧纹状体内。我们观察了正常大鼠、海姆-帕金森病大鼠的多巴胺能神经递质D_1、D_2受体和多巴胺摄取部位的变化。移植后，D2和D2多巴胺能受体恢复正常。对照纹状体有非常密集的多巴胺能摄取部位，与马吲哚结合。在同侧损伤侧，多巴胺摄取部位消失。移植纹状体内可观察到移植物周围的多巴胺摄取部位。b.胆碱能移植物实例：大鼠左侧Mynert核内注射鹅掌酸制作痴呆动物模型。取胎脑基底节的少量胆碱能细胞悬液移植到动物模型的同侧皮质和/或纹状体内。体外宏观放射自显影显示，移植区有明显的M胆碱能受体表达。在这些研究中，强调了移植区胆碱能受体的诱导方式与多巴胺能神经元的不同。

A. Cases of Dopaminergic graft :Catecholamine producing cells (suspension of fetal mesencephalic implants cells) were transplanted in the ipsilateral striatum of rats with hemi-parkinsonism induced by injection of 6-OHDA on the left side of the substantia nigra. We investigated the alterations of the straital D1, D2 dopaminergic receptors and dopamine uptake sites of three groups of rats, i. e control, heim-parkinsonism rats. After grafting, straital D1 and D2dopaminergic receptors were normalized. There were very dense dopaminergic uptake sites in the control striatum by mazindol binding. On the ipsilateral lesion side of the straitum, dopamine uptake sites had disappeared. In the transplanted striatum, dopamine uptake sites were observed around of the graft.B. Cases of cholinergic Graft :The animal model for dementic state were made by the injection of ibotenic acid in the left Mynert nucleus of the rats. Small amount of cholinergic cells suspensions which were obtained from the basal ganglia of the fetus brains were transplanted in the ipsilateral cortex and/or striatum of the animal models. By using in vitro macro-autoradiography, it was shown that muscarinic cholinergic receptors were clearly induced in the grafted areas. In these studies, it is stressed that the modes of cholinergic receptor inductions in the grafted areas are different from those of the dopaminergic neurons.