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Receptor Analysis of the Cholinergic and Dopaninergic Neuronal Graft in the Central Nervous System-in Vitro Macro-Autoradiographical Investigation

中枢神经系统胆碱能和多巴胺能神经元移植物的受体分析-体外宏观放射自显影研究

基本信息

批准号：
01570814
负责人：
MIZUKAWA Kiminao
金额：
$ 1.34万
依托单位：
Okayama University Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

A. Cases of Dopaminergic graft :Catecholamine producing cells (suspension of fetal mesencephalic implants cells) were transplanted in the ipsilateral striatum of rats with hemi-parkinsonism induced by injection of 6-OHDA on the left side of the substantia nigra. We investigated the alterations of the straital D1, D2 dopaminergic receptors and dopamine uptake sites of three groups of rats, i. e control, heim-parkinsonism rats. After grafting, straital D1 and D2dopaminergic receptors were normalized. There were very dense dopaminergic uptake sites in the control striatum by mazindol binding. On the ipsilateral lesion side of the straitum, dopamine uptake sites had disappeared. In the transplanted striatum, dopamine uptake sites were observed around of the graft.B. Cases of cholinergic Graft :The animal model for dementic state were made by the injection of ibotenic acid in the left Mynert nucleus of the rats. Small amount of cholinergic cells suspensions which were obtained from the basal ganglia of the fetus brains were transplanted in the ipsilateral cortex and/or striatum of the animal models. By using in vitro macro-autoradiography, it was shown that muscarinic cholinergic receptors were clearly induced in the grafted areas. In these studies, it is stressed that the modes of cholinergic receptor inductions in the grafted areas are different from those of the dopaminergic neurons.

A.多巴胺能移植病例：将产儿茶酚胺细胞（胎中脑植入细胞悬液）移植到黑质左侧注射6-OHDA诱导的偏侧帕金森病大鼠同侧纹状体。本实验观察了三组大鼠纹状体多巴胺D1、D2受体和多巴胺摄取部位的变化。e对照组，帕金森病组。移植后，纹状体D1和D2多巴胺能受体恢复正常。在对照纹状体中，马吲哚结合的多巴胺能摄取位点非常密集。在同侧病变侧的纹状体，多巴胺摄取部位消失。在移植的纹状体中，在移植物周围观察到多巴胺摄取位点。胆碱能神经移植术例：采用鹅膏蕈氨酸注射于大鼠左侧Mynert核造成痴呆动物模型。取胎脑基底节区的胆碱能细胞悬液，移植于动物模型同侧皮质和/或纹状体。通过使用体外宏观放射自显影术，表明在移植区域明显诱导了毒蕈碱胆碱能受体。在这些研究中，它强调，在移植区的胆碱能受体诱导的模式是不同的多巴胺能神经元。