The Sensitivity Test for Human Urinary Tract Malignancies Making of use of the Chick Embryo Method using Chorioallantoic Membrane

绒毛尿囊膜鸡胚法对人类尿路恶性肿瘤的敏感性试验

• 批准号: 01570882
• 负责人: UCHIBAYASHI Tadao
• 金额: $ 1.09万
• 依托单位: Kanazawa university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570882/
• 关键词: Sensitivity test; Urinary tract malignancies; Chick embryo method; MTT method; Molecular biology; 温熱治療; 感受性試験; 特異的DNA増幅法

In sensitivity tests of chemotherapy alone, hyperthermia alone, or a combination of the two against human urinary tract malignancies making use of the chick embryo method using chorioallantoic membrane, measurement of the weight of the tumor mass was considered to be an effective method of evaluating the antitumor effect. However, in cases in whicha a large part of the tumor is necrotic, this evaluation method did not provide reliable results. At present, using the the MTT method, more accurate quantitative results have been obtained. As a sensitivity test for metastatic tumors using continuously cultured cell lines, DNA is extracted from the metastatic organs of a fertilized chick embryo, specific DNA amplification and immuno-electro-phoresis are performed, and the DNA content is measured. By this method, it was shown that is possible to quantitatively evaluate the anti-tumor effect of various treatment methods.

在用鸡胚绒毛膜尿囊膜法对人尿路恶性肿瘤进行单独化疗、单纯热疗或两者联合治疗的敏感性试验中，测量肿瘤重量被认为是评价其抗肿瘤效果的有效方法。然而，在肿瘤大部分是坏死性的病例中，这种评估方法并不能提供可靠的结果。目前，利用四甲基偶氮唑盐比色法已获得了较为准确的定量结果。为了用连续培养的细胞系检测转移瘤的敏感性，从受精鸡胚胎的转移器官中提取DNA，进行特异性DNA扩增和免疫电泳法，并测定DNA含量。结果表明，该方法可以定量评价各种治疗方法的抗肿瘤效果。

项目成果

打林 忠雄,他: "鶏卵法による温熱と抗癌剤併用効果の検討" 日本泌尿器科学会雑誌. 80. 17-21 (1989)

Tadao Uchibayashi 等人：“使用鸡蛋法检查热疗和抗癌药物的联合效果”日本泌尿外科协会杂志 80. 17-21 (1989)。

T.Amano et al.: "A combined therapy of hyperthermia and tumor necrosis factor for nude mice bearing KKー47 bladder cancer" J.Urol.144. 370-374 (1990)

T.Amano 等人：“热疗和肿瘤坏死因子联合治疗患有 KK-47 膀胱癌的裸鼠”J.Urol.144 (1990)。

A. Nishino et al.: "A study of lymphocyte subsets in patients treated with regional hyperthermia for advanced urological malignancies" Jpn. J. Hyperthermic Oncol. 5. 27-36 (1989)

A. Nishino 等人：“对接受局部热疗治疗晚期泌尿系统恶性肿瘤的患者淋巴细胞亚群的研究”Jpn。

S. Lee etal.: "A study of local blood flow in renal cancer and normal tissue using a hydrogen gas clearance method" Jpn. J. Hyperthermic Oncol.5. 375-379 (1989)

S. Lee 等人：“使用氢气清除方法研究肾癌和正常组织的局部血流”Jpn。

T.Uchibayashi: "Responses of Tumor Cell Lines and Surgical Specimens Transplanted onto chorioallantoic Membrare of Chick Embryo to Anticancer Agents in Combination with/without Hyperthermia" Hyperthermic Oncology,'88,Taylor and Francis Publishing,Edited b

T.Uchibayashi：“肿瘤细胞系和移植到鸡胚绒毛尿囊膜上的手术标本对结合/不结合热疗的抗癌药物的反应”热疗肿瘤学，88，泰勒和弗朗西斯出版，编辑