Analysis of Carbohydrate Antigen Involving in sterility

众所周知，血清中含有精子固定抗体的女性很少能怀孕，尽管通过常规医学检查，她们的生殖器官没有异常。一种单抗(MAb)2H12。用人绒毛膜癌细胞株JEG-3免疫BALB/C小鼠。HAb2Hl2具有较强的精子固定化活性，能与硫脂和半脂发生反应。单抗2RI2的精子固定化活性可被硫脂或半脂明显吸收，而用人精子或精浆免疫制备的其他几种精子固定化单抗不能被相同的磺基糖脂吸收。不明原因不孕症妇女血清中的精子固定抗体也能被硫脂或半脂脂很好地吸收。单抗2HI2偶联免疫微球(MAb 2HI2-IMBS)可与活动精子结合。单抗2Hl2-IMBS与精子的结合可被单抗2Hl2或含有精子固定抗体的妇女血清竞争性抑制，但不能被正常妇女血清或其他几种精子单抗与人精子结合。这些结果表明，女性血清中的精子固定抗体是针对精子膜上3-0-磺基半乳糖残基的抗体。

It is well known that very few women who possess sperm immobilizing antibodies in their sera can conceive, though there are no abnormalities in their reproductive organs by routine medical examinations. A monoclonal antibody (MAb) 2Hl2. was produced by immunizing BALB/C mouse with human choriocarcinoma cell line JEG-3. The HAb 2Hl2 showed strong sperm immobilizing activities and reacted to sulfatide and seminolipids. The sperm immobilizing activities of MAb 2RI2 were clearly absorbed with sulfatide or seminolipid but several other sperm immobilizing MAbs that were made by immunization with human speria or seminal plasma could not be absorbed with the same sulfoglycolipids. The sperm immobilizing antibodies in the sera of sterile women with unknown cause were also absorbed well with sulfatide or seminolipid. The MAb 2HI2 conjugated immunobeads (MAb 2Hl2-IMBs) bound to motile sperm. This binding of MAb 2Hl2-IMBs to sperm was competitively inhibited either by MAb 2Hl2 or women's sera containing sperm immobilizing antibodies, but was not by normal women's sera or several other sperm immobilizing MAbs to human sperm. These results suggest that the sperm immobilizing antibody in women's sera is antibody against 3-0-sulfogalactose residue of seminolipid on the sperm membrane.