Production of anti-idiotype antibody against human monoclonal sperm immobilization antibody

抗人单克隆精子固定化抗体抗独特型抗体的制备

• 批准号: 01571238
• 负责人: SHIGETA Minoru
• 金额: $ 1.22万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571238/
• 关键词: Anti-idiotype antibody; Vaccine; Antisperm Autibody; 避妊ワクチン

Anti-idiotipic antibodies in mice against human monoclonal sperm immobilization antibody (H6-3C4) were produced to analyze the possibility for contraceptive vaccine by using anti-idiotype antibodies for internal image of antigen of sperm immobilization in animals and human. Sera from mice immunized with H6-3C4 were absorbed with human gamma globulin and purified antisera (AB2) were confirmed to react the H6-3C4. AB2 neutralized both sperm immobilizing activity of H6-3C4 and En46-A4 to human sperm. AS En46-A4 is class switched human SI-ab (IgGl) from H6-3C4 (IgM) by recombinant DNA technology, both antibodies were used same V gene for immunoglobulin. Then these have same antigen binding specificity (paratope) on the differrent class of immunoglobulin.Sera from rats and rabbit immunized with AB2 showed significant human sperm immobilization activity. These data suggested that AB2 contained internal image of sperm immobilizing antigen for rat and rabbit and could be suitable candidate for contraceptive vaccine instead of purified sperm antigen in animals and human.Then, we produced murine monoclonal anti-idiotype antibody against Mab H6-3C4 (Mono AB2) in order to apply for human. Four Mono AB2s were specific for H6-3C4 and one Mono AB2 had neutrizing activity of sperm immobilizing activity of H6-3C4.Rat antiserum against Mono AB2 had no sperm immobilizing activity for human sperm. Now we were making another murine Mono AB2.

用抗人精子固定化单克隆抗体（H6- 3C 4）制备小鼠抗独特型抗体，分析其对动物和人精子固定化抗原的内成像，作为避孕疫苗的可能性。用人γ球蛋白吸收来自用H6- 3C 4免疫的小鼠的血清，并证实纯化的抗血清（AB 2）与H6- 3C 4反应。AB 2中和H6- 3C 4和En 46-A4对人精子的精子固定活性。由于En 46-A4是通过重组DNA技术从H6- 3C 4（IgM）进行类别转换的人SI-ab（IgGl），两种抗体使用相同的免疫球蛋白V基因。AB_2免疫的大鼠和家兔血清均显示出明显的人精子固定活性。本研究制备了鼠抗单克隆抗体H6- 3C 4（Mono AB 2），并将其应用于人。4株Mono AB 2对H6- 3C 4有特异性，1株Mono AB 2对H6- 3C 4有中和精子的活性，而抗Mono AB 2的大鼠抗血清对人精子无固定活性。现在我们正在制造另一种鼠单核细胞AB 2。

项目成果

M.Shigeta,K.Sakata,S.Isojima: "Production and characterization of anfi-idiotypic antibody (AB2) against human monodonal sperm immobilzation antibody H6-3C4 (AB1)" Proceeding of the 5th Annual Meeting of Japan Society for Basic Reproductive Immunology and

M.Shigeta、K.Sakata、S.Isojima：“针对人单克隆精子固定化抗体 H6-3C4 (AB1) 的抗独特型抗体 (AB2) 的生产和表征”日本基础生殖免疫学会第五届年会论文集

M.SHIGETA: "Idiotype Cross-reactivity of Sperm immobilizing antibodies in infertile patient sera,which was difined with monodonal antibodies" Journal of Reproductive Immunology. Supplement. 48 (1989)

M.SHIGETA：“不育患者血清中精子固定抗体的独特型交叉反应性，用单克隆抗体确定”《生殖免疫学杂志》。

M. SHIGETA ; K. SAKATA ; S. ISOJIMA: "Production and characterization of anti-idiotypic antibody (AB2) against human monodoual sperm immobilization amtibody H6-3 C4 (AB1)" Proceedings of the 5th Annual Meeting of Japan society for Basic Reproductive immun

重田先生；