Production of anti-idiotype antibody against human monoclonal sperm immobilization antibody

用抗独特型抗体对动物和人精子固定抗原进行内成像，制备了抗人精子单抗(H6-3C4)的小鼠抗独特型抗体，以分析避孕疫苗的可能性。用人丙种球蛋白吸附H6-3C4免疫的小鼠血清，纯化的抗血清(Ab2)与H6-3C4发生反应。AB2可中和H6-3C4和En46-A4对人精子的固定作用。由于En46-A4是用重组DNA技术从H6-3C4(IgM)转换型的人SI-ab(IgG1)，所以两种抗体使用相同的V基因作为免疫球蛋白。这些抗体对不同类别的免疫球蛋白具有相同的抗原结合特异性(对位)。免疫大鼠和兔的血清显示出显著的人精子固定化活性。这些数据表明，Ab2含有大鼠和兔精子固定抗原的内图像，可以替代纯化的精子抗原用于动物和人的避孕疫苗。然后，我们制备了抗单抗H6-3C4的鼠源性抗独特型单抗(Mono Ab2)，以用于人类。4个Mono AB2对H6-3C4具有特异性，1个Mono AB2具有H6-3C4精子固定活性的中和活性，大鼠抗Mono AB2抗血清对人精子无固定活性。现在，我们正在制作另一种小鼠单核细胞AB2。

Anti-idiotipic antibodies in mice against human monoclonal sperm immobilization antibody (H6-3C4) were produced to analyze the possibility for contraceptive vaccine by using anti-idiotype antibodies for internal image of antigen of sperm immobilization in animals and human. Sera from mice immunized with H6-3C4 were absorbed with human gamma globulin and purified antisera (AB2) were confirmed to react the H6-3C4. AB2 neutralized both sperm immobilizing activity of H6-3C4 and En46-A4 to human sperm. AS En46-A4 is class switched human SI-ab (IgGl) from H6-3C4 (IgM) by recombinant DNA technology, both antibodies were used same V gene for immunoglobulin. Then these have same antigen binding specificity (paratope) on the differrent class of immunoglobulin.Sera from rats and rabbit immunized with AB2 showed significant human sperm immobilization activity. These data suggested that AB2 contained internal image of sperm immobilizing antigen for rat and rabbit and could be suitable candidate for contraceptive vaccine instead of purified sperm antigen in animals and human.Then, we produced murine monoclonal anti-idiotype antibody against Mab H6-3C4 (Mono AB2) in order to apply for human. Four Mono AB2s were specific for H6-3C4 and one Mono AB2 had neutrizing activity of sperm immobilizing activity of H6-3C4.Rat antiserum against Mono AB2 had no sperm immobilizing activity for human sperm. Now we were making another murine Mono AB2.