Cooperative studies of seroepidemiology and pathogenesis of Viral and parasitie diseases in Okinawa and Thailand

冲绳和泰国病毒和寄生虫病血清流行病学和发病机制的合作研究

• 批准号: 02045036
• 负责人: FUKUNAGA Toshihiko
• 金额: $ 3.2万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02045036/
• 关键词: Japanese encephailtis; Dengue hemerrhgic fever; Cross immune response in flavivirus infections; RT-PCR; Expressed protein by baculovirus system; Heres simplex virus type 2; Strongyloidiasis; Serodiagnosis of strongyloidiasis; 糞線虫症の血清学的診断法; 単純ヘルペス

The cooperative study consists of three fields : 1. Flavivirus infections, specifically Japanese encephalitis(JE) and dengue hemorrhagic fever(DHF). 2. Herpes simplex virus type 2(HSV 2) infection. 3. Strongyloidiasis.Results obtained are described briefly as follows :1. Cross immune response between JE and DHF was examined by neutralization(N) tests. When individuals with pre-existing N antibody to JE or dengue virus were infected sequentially with dengue or JE virus, significant increases of N titer were observed not only to the causative virus but also to the previously infected virus at the frequency of 35%-40%. Thus, even the N test could not always identify the causative virus in re-infection of flaviviruses.The truncated dengue-4 E protein produced by a recombinant baculovirus was wxamined for the possibility of diagnostic antigen to differentiate dengue and JE infection. Results indicated that the expressed protein was a useful diagnostic antigen in differentiation of dengue in … More fection from JE with slightly lower specificity than N test.RT-PCR method was examined for the early diagnosis of DHF. Appropriate selections of primers for RT-PCR resulted in a useful identification method of 4 serotypes of dengue virus in comparison with virus isolation.2. HSV 2 isolates in Okinawa and Chiang Mai were analyzed by the restriction endonuclease cleavage patterns and virulence to mice. Some cleavage patterns characteristic to each regional isolate were observed. The Okinawan isolates showed higher, intermediate and lower virulence while the Chiand Mai isolates showed only intermediate virulence to mice by peripheral injection3. Field study on strongyloidiasis in Chiang Mai gave positive rates of 46.3% in adult villagers and 6.9% in school children.The newly developed ELISA for strongyloidiasis gave well-corresponding results with those of the stool culture examination for the Okinawan samples but did not for the Chiang Mai samples, possibly due to cross reactions caused by co-infections with other intestinal parasites such as Opisthorchis viverrini and hookworm, which were found to be highly prevalent in the Chiang Mai area. Less

合作研究包括三个领域：1.合作研究；黄病毒感染，特别是日本脑炎（JE）和登革出血热（DHF）。2. 单纯疱疹病毒2型（HSV 2）感染。3. 类圆线虫病。所得结果简述如下：1。用中和(N)试验检测乙脑与DHF的交叉免疫反应。当已有乙脑或登革热病毒N抗体的个体连续感染登革热或乙脑病毒时，不仅对致病病毒，而且对先前感染的病毒的N滴度均显著升高，频率为35% ~ 40%。因此，在黄病毒再感染中，即使用N试验也不能总能识别出致病病毒。研究了重组杆状病毒产生的截短的登革热- 4e蛋白作为区分登革热和乙脑感染的诊断抗原的可能性。结果表明，该蛋白是一种有效的登革热诊断抗原，其特异性略低于N试验。采用RT-PCR方法对DHF进行早期诊断。选择合适的引物进行RT-PCR，与分离的登革病毒进行比较，得到了一种有效的登革病毒4种血清型的鉴定方法。用限制性内切酶裂解模式和对小鼠的毒力分析了冲绳和清迈地区HSV 2分离株。观察到各区域分离物具有一定的解理模式。冲绳分离株外周注射对小鼠表现出较高、中等和较低的毒力，而清迈分离株仅表现出中等毒力。清迈地区实地调查结果显示，成年村民蛲虫病阳性率为46.3%，学龄儿童阳性率为6.9%。新开发的圆线虫病ELISA检测结果与冲绳样本的粪便培养检测结果吻合良好，但与清迈样本的结果不一致，这可能是由于与清迈地区高度流行的其他肠道寄生虫（如viverrini Opisthorchis和钩虫）共同感染引起的交叉反应。少

项目成果

Sato, Y. et al.: "Application of enzyme-linked immunosorbent assay for mass examination for strongyloidiasis in Okinawa, Japan" International Journal for Parasitology. 20(8). 1025-1029 (1990)

Sato, Y. 等人：“酶联免疫吸附测定在日本冲绳类圆线虫病大规模检查中的应用”国际寄生虫学杂志。

Yoshitake, H. et al.: "Seroepidemiology of herpes simplex virus and restriction endonuclease cleavage analysis of herpes simplex virus type 2 in Okinawa" Acta Pathol. Jnp.41. 24-30 (1990)

Yoshitake, H. 等人：“冲绳单纯疱疹病毒的血清流行病学和 2 型单纯疱疹病毒的限制性核酸内切酶裂解分析”Acta Pathol。

Y.MAKINO et al.: "Potential use of baculobirus-expressed dengue-4 E protein as a diagnotic antigen in regions endemic for dengue and Japnese encephalitis" American Journal of Tropical Medicine and Hygiene. 45. 636-646 (1991)

Y.MAKINO 等人：“杆状病毒表达的登革热 4 E 蛋白作为登革热和日本脑炎流行地区的诊断抗原的潜在用途”《美国热带医学与卫生杂志》。

SATO,Y.et al.: "Application of enzyme-limked immunosorbent assay for mass examinaion of strongyloidiasis in Okinawa,Japan" International Journal for Parasitology. 20(8). 1025-1029 (1990)

SATO,Y.et al.：“酶联免疫吸附测定在日本冲绳类圆线虫病大规模检查中的应用”国际寄生虫学杂志。

Y. Makino et al.: "Potential use of a baculovirus-expressed dengue-4 E protein as a diagnostic antigen in regions endemic for dengue and Japanese encephalitis" American Journal of Tropical Medicine and Hygiene. 45. 636-646 (1991)

Y. Makino 等人：“杆状病毒表达的登革热 4 E 蛋白作为登革热和日本脑炎流行地区的诊断抗原的潜在用途”《美国热带医学与卫生杂志》。