Studies on the requlatory mechanisms of renal glomerular and tubular functions.

肾小球和肾小管功能调节机制的研究。

• 批准号: 02404036
• 负责人: KUROKAWA Kiyoshi
• 金额: $ 20.99万
• 依托单位: Faculty of Medicine, University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02404036/
• 关键词: mesangial cells; transforming growth factor beta (TGF-beta); platelet activating factor; endothelin; calcium; tubulo-glomerular feedback; バゾプレッシン; プロスタグランディン; 副甲状腺ホルモン; 近位尿細管; ホルモンレセプター; TGFーβ; エンドセリンーI; クロライド; PCR; レセプタ-; PAF(血小板刺激因子); 不

The major findings we obtained in this research project are as follows. 1) Mesangial cell expressed TGF-beta mRNA and its expression is stimulated by the addition of fetal calf serum and protein kinase C.Almost all the TGF-beta produced by mesangial cell and released into condition medium is in a latent form. Exogenously added TGF-beta inhibited mesangial cell growth. 2) A single class of PAF receptor is present in the quinea pig kidney which is most abundant in the cortex 3) Cloned renal tubule cells, LLC-PK1 cells, express a single entity of PAF receptor mRNA and PAF induced a dose-dependent calcium transients in LLC-PK1 cells. 4) Endothelin-1 dose-dependently increased [Ca^<++>]i both in cortical and medullary collecting duct. Endothelin-1 mRNA was found to be expressed only in cortical and medullary collecting ducts in addition to glomeruli and endothelin-1 binding occurs significantly in glomeruli and collecting tubules. 5) We proposed our hypothesis concerning the transcellular signal transduction in tubulo-glomerular feedback based on the observations we obtained in cultured mesangial cells.

我们在本研究项目中获得的主要发现如下。1)系膜细胞表达转化生长因子-βmRNA，其表达受胎牛血清和蛋白激酶C的刺激。系膜细胞产生的转化生长因子-β几乎全部以潜伏状态释放到条件培养液中。外源性加入转化生长因子-β可抑制系膜细胞的生长。3)克隆的肾小管细胞LLC-PK1细胞表达单一的PAF受体mRNA，PAF可诱导LLC-PK1细胞发生剂量依赖性钙瞬变。4)Endothelin-1可剂量依赖性地增加皮质和髓质集合管[Ca~(2+)]i。除肾小球外，ET-1mRNA仅在皮质和髓质集合管中表达，且在肾小球和集合管中有明显的结合。5)基于对培养的肾小球系膜细胞的观察，我们提出了关于肾小管-肾小球反馈中跨细胞信号转导的假说。

项目成果

S.Taniguchi: "Nephron distribution of platelet activating factor(PAF)reseptor mRNA detected by plymerase chain reaction(PCR)." J.Am.Sci.Nephrol.2(3). 465 (1991)

S.Taniguchi：“通过聚合酶链式反应 (PCR) 检测血小板激活因子 (PAF) 受体 mRNA 的肾单位分布。”

K.M, Asef Jamil: "Expression of platetet activating factor receptor in renal tubular cell line (LLC-PK1)" Biochem.Biophys.Res.Comm.187. 767-772 (1992)

K.M、Asef Jamil：“肾小管细胞系 (LLC-PK1) 中血小板激活因子受体的表达”Biochem.Biophys.Res.Comm.187。

T.Okuda他: "Demononstration of platelet actviating factor recepttor in guinea pig kidney." Biochem Biophys Res Comm.177. 54-60 (1991)

T. Okuda 等人：“豚鼠肾脏中血小板激活因子受体的演示。”Biochem Biophys Res Comm.177 (1991)。

H.Mathunaga,T.Okuda,K.Kurokawa: "Ionic cannel analysis of cultured rat mesangial cells.The Frontiers of Nephrology." edited by R.W.Berliner,N.Honda,K.J.Ullrich.Elsevier Science Publishers B.V., (1992)

H.Mathunaga、T.Okuda、K.Kurokawa：“培养的大鼠系膜细胞的离子通道分析。肾病学前沿”。

K.Kurkawa: "Cholride conductance of mesangial cells." Contrib.Nephrol.95. 76-81 (1991)

K.Kurkawa：“系膜细胞的氯离子电导。”