Three-dimensional molecular status of bone morphogenesis as revealed from molecular biological analyzes.

例如，脊椎动物腿的发育过程完全编码在肢芽组成的遗传信息中，腿的原基。在骨形态发生过程中，遗传信息在精确的时空控制下被表达，因此程序过程与发育过程中表观遗传表达的位置信息协同进行。本研究项目旨在揭示骨形态发生的三维分子状态，目前已通过两种不同的实验系统在发育组织水平或相关骨细胞水平进行了推广。我们的分子分析侧重于：(1)所谓的形态原实体，承载着位置信息；(2)同源框基因表达的分子调控，导致体轴的建立，或胚胎发育过程中上皮间充质的相互作用；(3)生长/分化因子的动态分布模式，特别是在肢芽中以三维方式释放，定向细胞-细胞分化相互作用，最后，(4)神经元与骨形态发生联系的分子机制。本研究结果初步阐明了脊椎动物胚胎发育过程中骨形态发生的基本三维分子状态。

The developing process of vertebrate legs, for example, is wholly encoded in the genetic information comprised by the limb buds, the leg primordia. During the bone morphogenesis, the genetic information is expressed under precise tempo-spatial control, thus the programd process proceeds in cooperation with positional information epigenetically expressed in the course of development.The present research project, aiming at revealing three-dimensional molecular status of bone morphogenesis, has sofar been promoted either at developing tissue level or the relevant bone cell level with two different experimental systems. The one is enchondral ossification system such as the limb bud development, and the other intramembraneous ossification system such as craniofacial development.Our molecular analyzes have focused on : (1)substantial entity of so-called morphogen, bearing positional information, (2)molecular control of homeobox gene expression leading to establishment of body axs, or of epitheliomesenchymal interaction during embryonic development, (3)dynamic distribution pattern of growth/differentiation factors, specifically released in three-dimensional fashion inside the limb buds, which orientates the cell-cell differentiating interaction, and finally, (4)molecular mechanism of neuron association of bone morphogenesis.Our results sofar obtained primarily elucidate basic three-dimensional molecular status of bone morphogenesis during development of vertebrate embryos.