A High-Pereormance Ultraviolet Apparatus

高性能紫外线仪

• 批准号: 02554018
• 负责人: HARADA Issei
• 金额: $ 9.54万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02554018/
• 关键词: UVRR spectroscopy; Raman spectroscopy; Fore-prism spectrometer; UV spectrometer; Stray light; CCD; Multichannel detector; Proteins; 紫外共鳴ラマン; CCD検出器; 紫外ラマン分光装置

A high-throughput and low stray-light multichannel ultraviolet Raman apparatus has been constructed. The apparatus is a Czerny Turner type single polychromator [Jasco, f = 575 mm, (F 6.3) ; a holographic grating, 4320 gr/mm (efficiency, 30% at 220 nm)] equipped with a fore-prism spectrometer (a Czerny Turner mount ; a synthetic 30ﾟ quartz prism, f = 200 mm ; reciprocal linear dispersion, ca. 1000 cm^<-1> at 240 nm) and a liquid-N_2 cooled CCD detector (Princeton Instruments, UV enhanced LN/CCD1152). The rpism spectrometer is used as a short-wavelength cut filter to reject the Rayleigh scattering by properly adjusting the exit slit width. The light passing through the filter spectrometer is further dispersed by the single grating polychromator and focused onto the CCD detector. The spectral range per 1152 channels is 1470 cm^<-1> at 240 nm (1.3 cm^<-1> per pixel of 22.5-muM width). UV radiation in the 213-266-nm range was generated by frequency conversion of 1064-nm pulses from a Nd : YAG laser (Quanta Ray DCR3G) operated at a 30-Hz repetition rate. For a suspension of bacteriorhodopsin, which is a retinoid membrane protein, spectra of high S/N ratios were obtained without saturation of the Raman bands and photochemical reaction.

研制了一台高通量、低杂散光的多通道紫外拉曼光谱仪。该装置是一个车尔尼·特纳型单多色仪[Jasco，f=575 mm，(F 6.3)；一个全息光栅，4320gr/mm(在220 nm处，效率为30%)]，配备了前棱镜光谱仪(车尔尼·特纳支架；合成30ﾟ石英棱镜，f=200 mm；倒易线性色散，约1000 cm；-1；棱镜光谱仪被用作短波切滤光器，通过适当调整出射狭缝宽度来抑制瑞利散射。通过滤光镜的光被单栅多色仪进一步分散，并聚焦到CCD探测器上。每1152个通道的光谱范围为240 nm处的1470 cm；-1(每22.5微米宽度的像素1.3 cm^-1)。在213-266 nm范围内的紫外光辐射是通过频率转换来自以30-Hz重复频率工作的ND：YAG激光器(量子射线DCR3G)的1064 nm脉冲而产生的。对于类维甲膜蛋白细菌视紫红质的悬浮液，获得了高的S/N比的光谱，而没有拉曼光谱的饱和和光化学反应。

项目成果

S. HASHIMOTO, A. TOYAMA, H. TAKEUCHI, T. IKEDA, and I. HARADA: "A New UV Raman Fore-Prism Multichannel Spectrometer"

S. HASHIMOTO、A. TOYAMA、H. TAKEUCHI、T. IKEDA 和 I. HARADA：“新型紫外拉曼前棱镜多通道光谱仪”

橋本 慎二: "UV Resonance Raman Study on Purple and Blue Membranes" Phorochem.Photobiol.

Shinji Hashimoto：“紫色和蓝色膜的紫外共振拉曼研究” Phorochem.Photobiol。