RESEARCH IN IMMUNOLOGICAL DIAGNOSIS AND VACCINATION THERAPY AGAINST BRAIN TUMORS USING ANTI-IDIOTYPIC ANTIBODY.

1)用人、大鼠和小鼠中常见抗原识别的单克隆抗体RS-11， Ab_1免疫制备大鼠抗独特型抗体（Ab_2）。免疫Ab_2可诱导小鼠产生抗独特型抗体（Ab_3），脾脏杀伤细胞活性增加。Ab_2免疫后移植大鼠胶质瘤细胞，与对照组相比，肿瘤生长受到抑制。这种抑制肿瘤生长的作用被抑制T淋巴细胞活性的抑制所夸大。2)利用识别人脑肿瘤相关抗原（TA226）的人单克隆抗体（CLN-IgG）制备抗独特型抗体（Idio-33）。Idio-33为β型抗独特型抗体。在重建人免疫系统的scid小鼠中，用Idio-33免疫治疗人胶质瘤，发现其对肿瘤生长有抑制作用。并在scid小鼠血清中诱导Ab_3。CLN-IgG治疗患者外周血淋巴细胞在Idio-33刺激下产生Ab_3。抗独特体在B淋巴细胞和辅助T淋巴细胞表面均有表达。

1), Rat anti-idiotypic antibody (Ab_2) was prepared by the immunization of monoclonal antibody (RS-11, Ab_1) recognized of the common antigen in species of human, rat and mouse. The anti-anti-idiotypic antibody (Ab_3) and the increased activity of killer cell in spleen were induced by the immunization of Ab_2. When the rat glioma cells were transplanted following the immunization of Ab_2, tumor growth was inhibited compared to controls. This tumor growth inhibiting effect was exaggerated by the inhibition of the activity of suppressor T lymphocytes.2), Anti-idiotypic antibody (Idio-33) was prepared using the human monoclonal antibody (CLN-IgG) recognized of the human brain tumor associated antigen (TA226). Idio-33 was beta type of anti-idiotypic antibody. In the scid mice with the reconstruction of human immune system, the inhibitory effect of tumor growth of human gliomas were found by the treatment with the immunization of Idio-33. And Ab_3 was induced in the sera of scid mice. The peripheral lymphocytes in the patients treated with CLN-IgG produced the Ab_3 by the stimulation with Idio-33. Anti-idiotope was expressed in the cell surface of B lymphocytes and also helper T lymphocytes.