Development of Analytical Instruments and Establishment of Analytical Method for Volatile Drugs and Poisons in Minute Amounts of Biological Samples

进行本研究是为了开发分析仪器并建立程序，能够准确和快速地分析挥发性物质（例如，乙醇、氯仿和甲苯），甚至在微量的生物样品中。主要研究结果如下：1.为了揭示铁磁合金重复使用的可能性，实验制作了特殊的铝胶囊，以防止铁磁合金分析后的污染和变质.在动物组织样品的乙醇，乙醚，氯仿和甲苯的基本实验中，通过脉冲加热法的测量值几乎相关的顶空法，分别。脉冲加热法可用于所有挥发物的测定.样本标本分为5份，每份标本同时在5个不同的机构进行检测：1） ...更多信息 在乙醇浓度测量值之间进行了比较;研究所A和B之间（相关系数（r）=0.943）; A和C之间（r=0.928）; A和D之间（r=0.990）; A和E之间（r=0.956），表明强正相关。2）还比较了乙醚、氯仿和甲苯的浓度。A与C之间的相关系数为0.601，A与E之间的相关系数为0.858。这些系数变化很大.这个问题需要进一步调查.用脉冲加热法和顶空法测定尸体血液和尿液中乙醇浓度。两种方法测定值的回归方程为：Y=0.117+0.993X（Y为脉冲法测定值; X为顶空法测定值; r=0.996;样本量，n=85），表明强正相关。从这些结果中，结果表明，脉冲加热法可用于微量样品中乙醇的快速、准确的定性和定量分析。通过测量乙醚、氯仿和甲苯获得的数据显示，不同研究所之间的差异很大。这需要进一步调查。脉冲加热对于快速定性筛选挥发物是非常有用的。少

The present research has been performed in order to develop analytical instruments and to establish the procedures, being capable of accurate and rapid analysis for volatile substances (e.g., ethanol, chloroform and toluene) even in minute amounts of biological specimens. The results obtained are as follows;1. In order to reveal the possibility of repeated usage of ferromagnetic alloys, special aluminum capsules were made experimentally to prevent contamination after analysis and deterioration of ferromagnetic alloys.2. In fundamental experiments with animal tissue samples for ethanol, diethylether, chloroform and toluene, the measured values by the pulse heating method almost correlated to those by the head space method, respectively. It was also revealed that the pulse heating method could be available for the determination of all of the volatiles examined.3. Sample specimens were divided into 5 portions, and the each specimen was examined simultaneously at 5 different institutes:1) … More In ethanol concentrations measured values were compared to each other; between institute A and B (correlation coefficient(r)=0.943); between A and C (r=0.928); between A and D (r=0.990); between A and E (r=0.956), indicating strong positive correlations.2)The concentrations of diethylether, chloroform and toluene were also compared. The correlation coefficient between A and C was 0.601 and between A and E, 0.858. These coefficients wide variations. The problem required a further investigation.4. Ethanol concentrations in the blood and urine obtained from autopsied cadavers were measured by the pulse Heating and the head space methods. The measured values by the two different methods showed the following regression formula : Y=0.117+0.993X (Y, the measured value by the pulse method; X, those by the head space method; r=0.996; sample size, n=85), indicating a strong positive correlationFrom these results, it has been revealed that pulse heating method was available for accurate and rapid analysis for ethanol in minute amounts of samples in both qualitative and quantitative analysis. The data obtained by measuring of diethylether, chloroform and toluene showed a wide variation among the different institutes. This required a further investigation. The pulse heating is, very useful for rapid qualitative screening of volatiles. Less