Freezing Process of Solution in Two Cells Separated by a Semipermeable Membrane

半透膜分隔的两个细胞中溶液的冷冻过程

• 批准号: 02650153
• 负责人: HINATA Shigeru
• 金额: $ 1.22万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650153/
• 关键词: Cryopreservation of Biological Organs; Cell's Cryoinjury; Semipermeable Membrane; Optical Fibre Refractometer; Refrigerating Curve; Double Diffusion Convection; Diffusion of Concentration; Dendrite; 樹状氷晶

An experimental study on mixing and freezing process of solution in two cells separated by a cellophane membrane was performed. A rectangular container of which the height, length and width were H=50 mm, L=30 mm and W= 38.4 mm, dividing into two cells by attaching the vertical membrane at the midplane of width was used. The left-hand side wall of the cell filled with the thick solution of NaCl-H2O of which the initial concentration is 20% and the right-hand side wall of the cell filled with the distilled water were abruptly cooled from the initial condition kept at temperature 40 C and were maintained at two constant but different temperatures, -20 C and -5 C respectively by pumping brine from two constant temperature baths through container behind the walls. The other walls were insulated except for the free surface. The refrigerating curves were measured by a horizontal rake of thermocouple which was positioned at the location corresponding to the elevation of H=25 mm from the bottom of the cells. The vertical distributions of concentration and temperature were obtained by using a salinity sensor. It was found that the stratification of solution in the cells was formed by mixing solute of solvent which passed through the membrane. The double diffusive interfaces were observed in the cell of thick solution side with going on a freezing process. It appeared that the growth rate of dendrites and the degree of super cooling in the cells separated by the cellophane membrane wall were small in comparison with the thin metal wall.

本文对两个玻璃纸膜分离池中溶液的混合和冻结过程进行了实验研究。使用高度、长度和宽度为H=50 mm、L=30 mm和W= 38.4 mm的矩形容器，通过在宽度的中平面处附着垂直膜而分成两个单元。将填充有初始浓度为20%的NaCl-H2O浓溶液的池的左侧壁和填充有蒸馏水的池的右侧壁从保持在40 ℃的初始条件突然冷却，并保持在两个恒定但不同的温度，通过将盐水从两个恒温槽泵送通过壁后的容器，分别在-20 ℃和-5 ℃下进行。除自由表面外，其他壁均为隔热壁。通过热电偶的水平耙测量制冷曲线，该热电偶位于对应于从电解槽底部的高度H=25 mm的位置处。利用盐度传感器获得了浓度和温度的垂直分布。结果表明，溶液的分层是由穿过膜的溶剂溶质混合而形成的。随着凝固过程的进行，浓溶液侧的胞内出现了双扩散界面。与薄金属壁相比，玻璃纸膜壁分隔的细胞中的枝晶生长速率和过冷度较小。

项目成果

S.Hinata,N.Himeno,M.Sakurai,K.Terao and H.Jida: "Mixing and Freezing Process of Solution in Two Cells Separated by a Membrane," Proc.of Third Int.Symp.on Heat Transfer,Bijing,China. (1992)

S.Hinata,N.Himeno,M.Sakurai,K.Terao 和 H.Jida:“溶液在由膜分隔的两个细胞中的混合和冷冻过程”，Proc.of Third Int.Symp.on Heat Transfer，北京，

S. Hinata, N. Himeno, M. Sakurai, K. Terao and H. Iida: ""Mixing and Freezing Process of Solution in Two Cells Separated by a Membrane"" Proc. of Third Int. Symp. on Heat Transfer. Beijing, China. (1992)

S. Hinata、N. Himeno、M. Sakurai、K. Terao 和 H. Iida：“用膜分隔的两个细胞中溶液的混合和冷冻过程”Proc。

日向 滋,姫野 修廣,桜井 正幸,寺尾 計彦,飯田 秀徳: "膜で仕切られたセル内の水溶液の凍結過程における濃度測定" 計測自動制御学会中部支部,第1回インテリジェントセンシング技術研究会講演論文集. 5-9 (1991)

Shigeru Hinata、Nobuhiro Himeno、Masayuki Sakurai、Keihiko Terao、Hidenori Iida：“在膜分隔的细胞中水溶液冷冻过程中的浓度测量”在学会中部分会第一届智能传感技术研究小组上发表的论文仪器与控制工程师卷 5-9 (1991)