Solubilization of Biological Substance by Using Water-Soluble Polymers

使用水溶性聚合物溶解生物物质

• 批准号: 02650651
• 负责人: INAMURA Isamu
• 金额: $ 1.28万
• 依托单位: Shimane University.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650651/
• 关键词: Water-Soluble Polymer; Protoporphyrin IX; Chlorophyllin (Cu Na salt); Chlorophyll a; beta-Carotene; Gel Chromatography; Absorption Spectrum; Aggregate; ビリルビン; 可溶化; モノマ-; クロロフィルa; βーカロチン; ポリビニルピロリドン; 複合体; 相互作用

1. Association Behavior of Protoporphyrin in Water and Aueous Poly(N-vinylpyrrolidone)Solutions :The association behavior of protoporphyrin IX was investigated at different pH by absorption spectroscopy and gel chromatography. Protoporphyrin IX existed as monomer in acidic water as aggregate in neutral water, and as dimer in basic water. Poly(N-vinylpyrr olidone)(PVP)tended to suppress dimerization and aggregation of protoporphyrin IX.2. Gel Chromatography of Chlorophyllin(Cu-Na salt)in Water and Aqueos Poly(N-vinylpyrrolidone)Solution :Gel chromatography of chlorophyllin(Cu-Na salt)in water was compared with that in aqueous PVPsolution. A shoulder at 430 nm in the absorption spectra was due to monomer of chlorophyllin(Cu-Na salt). PVP tended to suppress aggregation of chlorophyllin(Cu-Na salt).3. Interaction between Water-Soluble Polymer and Chlorophyll a, beta-Carotene :The treatment of ultrasonic wave had no effect on the wavelength and half band width of red peak of chlorophyll a. By gel chromatography, a red-shifted absorption band(>495 nm)of beta-carotene was not separated from the other bands of shorter wavelength.4. Solubilization of Bilirubin in Neutral Water by Using Ammonia :

1.原卟啉在水和聚N-乙烯吡咯烷酮水溶液中的缔合行为：用紫外吸收光谱和凝胶色谱研究了原卟啉IX在不同pH值下的缔合行为。原卟啉IX在酸性水中以单体形式存在，在中性水中以聚集体形式存在，在碱性水中以二聚体形式存在。聚（N-乙烯基吡咯烷酮）（PVP）倾向于抑制二聚化和聚集的原卟啉IX。叶绿素（铜钠盐）在水和聚（N-乙烯基吡咯烷酮）水溶液中的凝胶色谱：比较了叶绿素（铜钠盐）在水和聚（N-乙烯基吡咯烷酮）水溶液中的凝胶色谱。在吸收光谱中在430 nm处的肩峰是由于叶绿素的单体（Cu-Na盐）。PVP有抑制叶绿素（Cu-Na盐）聚集的趋势.水溶性高分子与叶绿素a、β-胡萝卜素的相互作用：超声波处理对叶绿素a红色峰的波长和半带宽没有影响。凝胶色谱分析发现，β-胡萝卜素的一个红移吸收带（>495 nm）与其它较短波长的吸收带没有分离.用氨在中性水中溶解胆红素：

项目成果

稲村 勇: "Gel Chromatography of Chlorophyllin (CuーNa salt) in Water and Aqueous Poly(Nーvinylpyrrolidone)Solution"

Isamu Inamura：“水和聚（N-乙烯基吡咯烷酮）水溶液中叶绿素（铜钠盐）的凝胶色谱法”