Genetic analysis of plasmid dependent prophage for molecular breeding in Bacillus thuringiensis
苏云金芽孢杆菌分子育种中质粒依赖性噬菌体的遗传分析
基本信息
- 批准号:02660051
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1991
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Bacillus thuringiensis which is well known for the production of parasporal inclusions(crystals)toxic for lepidopterous, dipterous and coleopterous insects plays an important role in the microbial control of pest insects. B. thuringiensis strain AF101, belonging to H-serotype 4a : 4b, was selected as the strain of low toxic to silkworm, but highly toxic to other lepidopterous pest insects. Strain AF101 was revealed to possess a single plasmid(pAF101)which contained crystal gene and the entire genome of temperate phage J7W-I. In this study, the genetic analysis of pAF101 in stain AFIOI was carried out for the purpose of developing the effective tool for the molecular breeding of B. thuringiensis.The physical map of pAF101 was constracted by digesting restriction enzymes. It was revealed that a part of the genome of temperate phage KK-88 which was also lysogenized phage in AF101 and had different induction system from phage J7W-l was located on pAF101. Furthermore, the induction system of these phages were examined to reveal that 34kdal and 50kdal of cellar proteins were repressor like proteins for J7W-l and KK-88, respectively. The crystal gene was cloned from pAF101- The location on pAF101 was detected to find that the crystal gene region was located between the regions of J7W-l phage genome' and KK-88 phage genome. According to these mentioned above, pAF101 was constrcted by mainly three genetical regions, such as two types of phage genome and crystal gene.
苏云金芽孢杆菌(Bacillusthuringiensis)是一种能产生对鳞翅目、双翅目和鞘翅目昆虫具有毒性的伴孢内含物(晶体)的细菌,在害虫的微生物防治中发挥着重要作用。B。苏云金杆菌菌株AF 101属于H-血清型4a:4 b,是对家蚕低毒,而对其它鳞翅目害虫高毒的菌株。菌株AF 101含有单一质粒pAF 101,该质粒含有J7 W-1的晶体基因和全基因组。本研究对AFIOI菌株的pAF 101基因进行了遗传分析,旨在为B的分子育种提供有效的工具。利用限制性内切酶对pAF 101的物理图谱进行了分析。结果表明,在pAF 101上定位了一部分温和噬菌体KK-88的基因组,该噬菌体也是AF 101中溶原化的噬菌体,与噬菌体J7 W-1具有不同的诱导系统。此外,对这两个菌株的诱导系统进行了研究,发现34 kdal和50 kdal的细胞蛋白分别是J7 W-1和KK-88的阻遏蛋白。从pAF 101中克隆了晶体基因,并对晶体基因在pAF 101上的定位进行了检测,发现晶体基因区位于J7 W-1噬菌体基因组和KK-88噬菌体基因组之间。根据上述结果,pAF 101主要由两种噬菌体基因组和晶体基因三个遗传区域所组成。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kohzo Kanda et al.: "Distribution of a plamid integrative prophage J7Wー1 among Bacillus thuringiensis type strain" FEMS Microbiology Letters.
Kohzo Kanda 等人:“苏云金芽孢杆菌型菌株中质粒整合前噬菌体 J7W-1 的分布”FEMS 微生物学快报。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kohzo Kanda et al.: "Partial integration of prophage KK-88 into a plasmid in Bacillus thuringiensis strain AF101" Journal of General Microbiology.
Kohzo Kanda 等人:“原噬菌体 KK-88 部分整合到苏云金芽孢杆菌菌株 AF101 的质粒中”普通微生物学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kohzo Kanda: "Distribution of a plasmid integrative prophage J7W-1 among Bacillus thuringiensis type strain" FEMS Microbiology Letters.
Kohzo Kanda:“质粒整合原噬菌体 J7W-1 在苏云金芽孢杆菌型菌株中的分布”FEMS 微生物学快报。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kohzo Kanda et al.: "Distribution of a plasmid integrative prophage J7W-1 among Bacillus thuringiensis type strain" FEMS Microbiology Letters.
Kohzo Kanda 等人:“苏云金芽孢杆菌型菌株中质粒整合性噬菌体 J7W-1 的分布”FEMS 微生物学快报。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kohzo Kanda: "Partial integration of prophage KK-88 into a plasmid in Bacillus thuringiensis" Journal of General Microbiology.
Kohzo Kanda:“前噬菌体 KK-88 部分整合到苏云金芽孢杆菌的质粒中”普通微生物学杂志。
- DOI:
- 发表时间:
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- 影响因子:0
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KANDA Kohzo其他文献
KANDA Kohzo的其他文献
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{{ truncateString('KANDA Kohzo', 18)}}的其他基金
The develeopment of novel host-vector system in Bacillus thuringiensis.
苏云金芽孢杆菌新型宿主载体系统的开发。
- 批准号:
05660053 - 财政年份:1993
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)