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Elucidation of Biosynthesis and Metabolism of Nicotinic Acid Related Compounds and Various Specific Physiological Functions of the compounds

烟酸相关化合物的生物合成和代谢及其各种特定生理功能的阐明

基本信息

批准号：
02660089
负责人：
TAGUCHI Hiroshi
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

1. Growth-prmoting Effect of Cinchomeronic Acid (CA)(1) On Radish Seeding : Maximum growth of the stem was observed at 10mM of CA and it was 2.18 times longer than that of control. Cell length was several times longer and cell width was about the half as compared to those of the control on microscopic obervation of the epidermal cells of radish stems. Indoleacetic acid slightly promoted the growth of radish seedings.(2) On Escherichia coli and Other Microorganisms : At 0.1-100mM of CA with E. coli B was most effective among various microorganisms (lactic acid bacteria, E. coli, Bacillus subtiis, yeast).2. Metabolism of Cinchomeronic Acid in Plants and Microorganisms No radioactive peak other than CA was observed in the extract from duckweed and E. coli B grown in the medium containing [3, 4, 7, 8-^<14>C] CA on radio HPLC analysis. Also no radioactive peak except CA was observed in the reaction mixture after incubating [^<14>C] CA with the cell-free extract from duckeweed and E. coli B. Hence, CA may not be metabolized in the these organisms.3. Function of Various Nicotinic acid-Related Compounds(1) On Cultured Animal cells : Almost all compounds added to the medium of 3 kinds of animal cells were inhibitory.(2) On Flowering Induction in Duckweed : Relationship between intrinsic nicotinic acid-, nicotinamide-, and NAD-contents and flowering induction was not found.4. Biosynthesis of Homarine in marine InvertebratesProposed pathway from glycine to homarine can not be supported with various investigations.

1.金鸡纳酸（CA）对萝卜幼苗的促生长作用：10 mM CA处理的萝卜茎生长量最大，是对照的2.18倍。在显微镜下观察萝卜茎表皮细胞，细胞长度是对照的几倍，细胞宽度约为对照的一半。吲哚乙酸对萝卜茎的生长有一定的促进作用。(2)对大肠杆菌和其他微生物：在0.1- 100 mM CA与E.大肠杆菌B的抑菌效果最好，其次是乳酸菌、大肠杆菌、乳酸杆菌。大肠杆菌、枯草芽孢杆菌、酵母）。金鸡纳酸在植物和微生物中的代谢浮萍和E.用放射性高效液相色谱法分析在含[3，4，7，8-^ C] CA的培养基中生长的大肠杆菌B<14>。此外，在将[^<14>C] CA与浮萍和E.大肠杆菌B。因此，CA可能不会在这些生物体中代谢。3.各种烟酸相关化合物的作用（1）对培养的动物细胞：添加到3种动物细胞培养基中的几乎所有化合物都是抑制性的。(2)浮萍成花诱导研究：未发现内源烟酸、烟酰胺和NAD含量与成花诱导的关系.海洋无脊椎动物中Homarine的生物合成从甘氨酸到Homarine的可能途径并没有得到各种研究的支持。