权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Microbial Fixation of CO_2 to Organic Compounds and Its Application to the Production of useful materials

CO_2对有机化合物的微生物固定及其在有用材料生产中的应用

基本信息

批准号：
02660119
负责人：
AOKI Kenji
金额：
$ 1.41万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660119/
关键词：
fixation of CO_2 aniline anthranilic acid autotrophic microorganism Rhodococcus biotin catechol 1, 2-dioxygenase catechol 2, 3-dioxygenase

项目摘要

When Rhodococcus erythropolis AN-13 grew on aniline, a fluorescent substance accumulated in the cultural fluid. It was obtained as crystals and identified as anthranilic acid (AnA). AnA was also produced from aniline following incubation with resting cells of the bacterium grown on aniline. Heated cells lost the activity to produce it, and aniline was essential for its production. The production of AnA was promoted by sodium bicapbonate ; when ^<14>C-sodium bicarbonate was added to the incubation mixture, ^<14>C-AnA formed. the optimal pH for AnA production by the resting cells was 7.0 to 7.5. These results suggest that microbial activities of R. erythropolis AN-13 catalyzed the formation of AnA from aniline.The production was investigated for 40 bacterial strains in the presence and absence of aniline. Resting cells of all aniline-assimilating R. erythropolis strains produced more AnA than those of other anilineassimilating bacteria. Resting cells of several non-aniline-assimilating s … More trains produced AnA in the absence of aniline. However, its production by these strains was much lower than that by the Rhodococcus strains. The production of AnA by cells of aniline-assimilating R. erythropolis AN-13 was promoted by aliphatic monocarboxylates, ATP, biotin and coenzyme A, and repressed by catechol analogues, N-ethylmaleimide and iodoacetate. on the other hand, its production by non-aniline-assimilating PseudoNonas sp. AN21 was repressed by glucose, mannose and some amino acids.Cell-free extracts prepared from R. erythropolis AN-13 cells retained the activity capable of producing AnA from aniline. The catalytic factor(s) in these extracts was partially purified by fractionation with streptomycin sulfate and anunonium sulfate and gel filtration on a column of Tyopearl HW55s. Further purification procedures of this catalytic factor(s) are in progress.A bacterial strain, FK-8-2, that metabolized aniline through a metacleavage pathway was isolated and identified as Pseudomonas. Pseudomonas sp. FK-8-2 produced much catechol 2, 3-dioxygenase in its cells. This enzyme was purified to homogeneity. The purified enzyme differed in chemical and catalytic properties from that obtained from Pseudomonas putida mt-2 growing on benzoate. Another aniline-assimilating bacteria producing isozymes of catechol 1, 2dioxygense in their cells were also isolated. These isolates will be useful materials for investigating on the bacterial production of AnA from aniline. Less

当红串红球菌AN-13在苯胺上生长时，荧光物质在培养液中积累。它以晶体形式获得，并被鉴定为邻氨基苯甲酸（AnA）。AnA也产生苯胺与静息细胞培养苯胺上生长的细菌。加热的细胞失去了生产它的活性，苯胺是其生产所必需的。碳酸氢钠可促进AnA的生成;当<14>向孵育混合物中加入14 C-碳酸氢钠时，形成14 <14>C-AnA。静息细胞产生AnA的最适pH为7.0 - 7.5。这些结果表明，R.红串菌AN-13催化苯胺合成AnA，考察了40株菌株在苯胺存在和不存在下的合成情况。所有苯胺同化R.红串菌产生的AnA比其他苯胺同化菌多。几种非苯胺同化物的静息细胞 ...更多信息在不存在苯胺的情况下生产AnA。然而，这些菌株的产量远低于红球菌菌株。苯胺同化R.脂肪族单羧酸盐、ATP、生物素和辅酶A对红串菌AN-13的生长有促进作用，而邻苯二酚类似物、N-乙基马来酰亚胺和碘乙酸盐对AN-13的生长有抑制作用。另一方面，葡萄糖、甘露糖和某些氨基酸抑制非苯胺同化的PseudoNonas sp.AN21的产酶。红串菌AN-13细胞保留了能够从苯胺产生AnA的活性。用硫酸链霉素和硫酸铵分级分离，并在Tyopolysis HW 55 s柱上凝胶过滤，部分纯化了这些提取物中的催化因子。分离到一株通过后裂解途径代谢苯胺的细菌FK-8-2，经鉴定为假单胞菌属（Pseudomonas）。假单胞菌FK-8-2产生大量邻苯二酚2，3-双加氧酶。将该酶纯化至均一。纯化的酶不同的化学和催化性能从恶臭假单胞菌MT-2苯甲酸盐上生长。还分离到一株能产生邻苯二酚1，2-双加氧酶同工酶的苯胺同化菌。这些菌株将为研究细菌利用苯胺生产AnA提供有用的材料。少