Bonding between the woody fibers by the oxidative coupling of lignin model compounds with phenoloxidase.

过氧化物酶和漆酶是催化酚醛羟基脱氢并形成羟基自由基的酶。exampIe。当酶作用于分离的酵素时。尽管一部分Iignin变成了低分子量物质。大部分木质素与高分子量物质交联。这种聚合反应是由漆酶作用形成的自由基之间的偶联反应。在木质素化过程中。据说，在纤维素和/或半纤维素之间提供的I -木质素前体。通过酶5UCh - Ss过氧化物的作用聚合。然后纤维之间的结合强度就提高了。在这项研究中。我们试图利用木质素、木质纤维和/或木质素前体上产生的自由基进行脱氢。然后木质纤维通过自由基偶联反应结合在一起。以竹笋为原料制备过氧化物。调查)。以川竹（Coriolus versicolor IFO-8482）为培养基制备漆酶。用过氧化物酶透析管法和漆酶滴加法进行脱氢反应。在结果中，它。确定了以香草酸为原料制备脱氢聚合物质的最佳工艺条件；PH值约为6。反应时间为24小时。丙烯酸含量较高。过氧化氢的活性约为1.5万个单位。30% H202的用量为8-1 nL。VA-DHP在热拉>(TMP)上沉淀吸附。然后用vA-DHP-TMP配合物制备双层粘结纸。在VA-DHP生产的最佳条件下，胶合强度（PS）最大。胶合纸的强度约为UA-DHP和TMP复合胶合纸强度的1.7倍。认为TMP与UA-DHP之间的偶联键是在TMP存在下进行脱氢聚合时形成的。然后得出纤维间的胶合强度。用脲醛脲和漆酶合成脱氢聚合物质，用漆酶活化木质纤维，改善纤维间的结合。结果表明，当TMP中的木质素被漆酶的作用激活时。然后通过过氧化物酶的作用将UA-DHP沉淀在叶片上。胶合板强度提高。将脱氢聚合反应应用于双盘精炼纸浆（DDRP）纤维板的制备。结果显示，当氧化酶和漆酶处理DDRP时。提高了板材的内粘接强度（IB）和耐水性。在上漆处理。w Less的改进

Peroxidase and laccase are the enzymes that catalyze the dehydrogenation of phenolic hydroxyl group and make up to phonoxy radicaI. For exampIe. when the Iaccase act to the isolated Iignin. although a part of Iignin change to low molecuIar-weight substancees. alarge portion of the lignin cross-linke to highe molecular-weight substanses. this polymerization reaction is the coupling-reaction between the radicals which are made up by the action of laccase. in the process of lignification. it is said that the I ignin-precursors, wich were 4 suppiied between cellulose and/or hemicellulose. polymerized by the actions of enzymes 5UCh Ss peroxidass. and then the bonding strength developed between the f ibers. In this study. we attempted that this dehydrogenation was made use the radicals were produced on the lign, in a woody fiber and/or on lignin-precursors. and then the woody fibers were bonded tgether through the radicalcoupling reaction. The peroxida5e waS PrOpared mom the bamboo shoots of … More Madake(Phyllastachys bambusoides Slob. et Zucc.). and laccase was prepared from the liquid-medium of Kawaratake(Coriolus versicolor IFO-8482). The dehydrogenation reactions were proceeded on the dialysis tube method with peroxidase and on the zut ropfuerfahren(the dropwise-additi on method)with laccase. In the results, Its. the optimum condit ions for the production of the dehydrogenative polymerization substance from the vanillic acid(UA-DHP)were was follows ; PH was about 6. reaction time was 24 hours. amount of veniIIic acid was more. activity of perox ida5e was about 15, 000 units. and the amount of 30% H202 WaS 8-1 nL. VA-DHP were precipitated and adsorbed on thermomechanical pull>(TMP). and then the vA-DHP-TMP complexes were used for the preparation or two-ply bonded paper. The plybonding strength(PS)of the paper were maximum on the same to the optimum condition of the VA-DHP production. The plybond strength were about 1.7 times of the strength of the plybonded paper to be made from the mixture of UA-DHP and TMP. It was thought that the coupling-bonds between the TMP and UA-DHP were formed when the vani Ilic acid were polymerized dehydrogenatively in the presence of TMP. and then the plybonding strength between the fibers was appeared. The production of dehydrogenative polymerization substance by the zutroptuerfahren6 with the laccase and the improvement of the bonding between the ribers by the activation of the woody fiber with the laccase. In the results, when the lignin in the TMP was activated by the function of laccase. and then the UA-DHP by the function of peroxidase were precipitated on the(ibors. the plybond strength were increased. The dehydrogenative polynerization reaction was applied to the manufacture of fiber board mom the double-disk refiner pulp(DDRP). In the results, when DDRP was treated by poxidase or laccase. the internal bonding strength(IB)and water-resistants of the boards were increased. In the laccase -treatment. the improvement of w Less